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FISH (fluorescence in situ hybridization) is routinely used to label features in planarians (Schmidtea mediterranea). The NIGHTSEA Model SFA Stereo Microscope Fluorescence Adapter can be added to just about any existing stereo microscope to create a practical system for screening samples for successful preparation prior to moving to higher resolution imaging techniques.
PIWI gene (marker for stem cells) labeled with FAM (fluorescein amidite). Specimens courtesy of Donald Von Kannon, laboratory of Dr. Wendy Beane, Western Michigan University.
All specimens below courtesy of Lauren Cote, Reddien Laboratory, Whitehead Institute, MIT
Collagen labeled with FITC (fluorescein isothiocyanate)
Now the same samples at higher magnification, imaged with the Keyence VHX-5000 with the NIGHTSEA fluorescence adapter.
Opsin and wntP-2 labeled with FITC