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Which NIGHTSEA excitation and emission combination should I use for my fluorophore (GFP, FITC, RFP…)?
What is the basis for the recommendations?
Table 1 – NIGHTSEA wavelength sets
Wavelength set names are sets based on the color of the excitation light, not on the color of the fluorescence that they will excite.
Designation | Excitation | Emission |
UV – Ultraviolet | 360 – 380nm | 415nm longpass |
VI – Violet | 400 – 415nm | 450nm longpass |
RB – Royal Blue | 440 – 460nm | 500nm longpass |
RB – Royal Blue | 440 – 460nm | 500 – 560nm bandpass |
CY – Cyan | 490 – 515nm | 550nm longpass |
GR – Green | 510 – 540nm | 600nm longpass |
Table 2 – Fluorophores and recommended NIGHTSEA wavelength sets
Key:
Fluorescent Protein/Dye | UV | VI | RB | CY | GR | Basis |
Alexa Fluor 488 | X | W | ||||
Alexa Fluor 555 | X | s | ||||
Alexa Fluor 568 | X | s | ||||
Alexa Fluor 594 | X | s | ||||
amCyan1 | X | s | ||||
Aquamarine | X | s | ||||
Calcein | X | W | ||||
Cerulean | X | s | ||||
CFP | X | W | ||||
Citrine | X | s | ||||
Clover | X | s | ||||
Cy3 | X | s | ||||
CyOFP1 | X | s | ||||
CyPet | X | s | ||||
DAPI | X | W | ||||
Dextran-FITC | X | s | ||||
Dextran-Texas Red | X | s | ||||
Dextran-TRITC | X | s | ||||
DiI | X | s | ||||
DsRed | 2 | 1 | W | |||
E2-Orange | X | s | ||||
EBFP | X | s | ||||
EBFP2 | X | s | ||||
ECFP | X | W | ||||
EGFP | X | W | ||||
eqFP670 | X | s | ||||
EYFP | X | W | ||||
FITC | X | W | ||||
Fluorescein | X | W | ||||
FusionRed | X | W | ||||
Fluorescent Protein/Dye | UV | VI | RB | CY | GR | Basis |
GFP | X | W | ||||
Hoechst | X | W | ||||
iFP1.4 | 1 | 2 | s | |||
KO1 | X | s | ||||
LSSmOrange | X | s | ||||
Lucifer Yellow | X | W | ||||
mAmetrine | X | s | ||||
mApple | X | s | ||||
mAzamiGreen | X | s | ||||
mBanana | X | s | ||||
mBeRFP | X | s | ||||
mCardinal | X | s | ||||
mCherry1 | X | W | ||||
mCitrine | X | s | ||||
mEmerald | X | s | ||||
mGarnet2 | X | s | ||||
mHoneydew | 1 | 2 | s | |||
MiCy | X | s | ||||
mKalama1 | X | s | ||||
mKate | X | s | ||||
mKate2 | X | s | ||||
mKeima | X | s | ||||
mKO2 | X | s | ||||
mKusabira-Orange | X | s | ||||
mMidoriishi-Cyan | X | s | ||||
mNeonGreen | X | s | ||||
mNeptune | X | s | ||||
mOrange | X | s | ||||
mOrange2 | X | s | ||||
Fluorescent Protein/Dye | UV | VI | RB | CY | GR | Basis |
mPapaya1 | X | s | ||||
mPlum | X | s | ||||
mRaspberry | X | s | ||||
mRFP1 | X | s | ||||
mRFP1.2 | X | s | ||||
mRuby | X | s | ||||
mScarlet | X | s | ||||
mStable | X | s | ||||
mStrawberry | X | s | ||||
mTagBFP | X | s | ||||
mTangerine | X | s | ||||
mTurquoise2 | 1 | 2 | s | |||
mUKG | X | s | ||||
mWasabi | X | s | ||||
RFP | 2 | 1 | W | |||
Rhodamine B | X | W | ||||
Sapphire | 2 | 1 | s | |||
Superfolder GFP | X | s | ||||
SYFP2 | X | s | ||||
TagBFP | 1 | 2 | W | |||
TagCFP | X | s | ||||
TagGFP2 | X | s | ||||
TagRFP | 2 | 1 | s | |||
TagRFP-2 | 2 | 1 | s | |||
TagYFP | X | s | ||||
tdTomato | 1 | 2 | W | |||
Texas Red | X | s | ||||
TRITC | X | W | ||||
Venus2 | X | s | ||||
YFP | X | W | ||||
Ypet | X | s | ||||
ZsGreen | X | s |
Table 2 Notes: