Questions? 800-523-5874 | [email protected]
- Prepmaster™ Specimen Preparation Robot
- TEM Grids
- TEM Window Grids
- Omniprobe Nanomanipulation Systems
- K-kit Wet "Liquid" TEM Kit
- Specimen Mounts
- SEM Specimen Holders
- Index and Finder SEM Grids
- SEM for Forensics
- SEM Sample Preparation Station Materials
- Cryogenic Personal Protection Equipment
- Cryo Dewars & Flasks
- Cryogenic Grids & Accessories
- Cryogenic Vials & Racks
- Cooling Chambers & Ice Baths
- Prepmaster™ Specimen Preparation Robot
- Laboratory Microwave Ovens
- LYNX II Automated Tissue Processor
- EMS Poly III
- Microtomes
- Tissue Slicers
- Rapid Immersion Freezer
- Heaters & Chillers
- SEM Cooling Stage
- Glow Discharge Systems
- Sputter Coaters & Carbon Coaters
- Stages
- Freeze Dryers
- Critical Point Dryers
- Cryo-SEM Preparation System
- Specimen Transfer Systems
- Decontaminators
- Desiccators
- Centrifuges
- Dry Baths
- Stirrers, Hot Plates
- Vortexers & Magnetic Mixers
- Rotators & Rockers
- Ovens & Incubators
- Vibration Isolation
- Air Sampling
- Vacuum Pumps
EMS Technical Tips
Controlling Background in Immunogold Labeling
Background Is Controlled by Three Independent Steps
1. Low Molecular Weight Block (before protein block)
Purpose: to inactivate residual fixative e.g. aldehydes, using
- amino acids such as glycin or lysin, or
- aldehyde inactivating compounds such as NaBH4 and NH2OH
2. High Molecular Weight Protein Block (before immunolabeling)
Purpose: to prevent stickiness to hydrophobic areas and domains with excessive positive charges based on multiple point interactions (high affinity protein binding capacity), using
- albumin
- normal serum
3. Incubation and Wash solution (during immunolabelling)
Purpose: to eliminate aspecific binding of immunoconjugates based on hydrophilic interactions ("oligo"point interactions) by competition, using acetylated albumin (AURION BSA-cā¢) in the incubation buffer.
For more information
Please refer to Immunogold Newsletter 1 and Newsflyer 1 for more information on the subject of background.