EMS Technical Tips

Blocking Solutions Application Instructions

Detailed information is provided on the package inserts.

Electron Microscopy

- On-Grid Labeling

Placing grids on top of drops of Blocking Solution arrayed on a sheet of Parafilm™ after they have been treated for aldehyde inactivation.

- Pre-embedding Labeling

Floating specimens in Blocking Solution, preferably on a rocking table.

Light Microscopy

- On-slide or -coverslip Labeling

Applying a few drops of Blocking Solution to cover the specimen.

- Bio Assays

Incubating specimens (e.g. immunoblot strips) in Blocking Solution on a rocking table in screw-cap sealed disposable tubes.

Additional Information

 

For additional information please see the Aurion Immunogold Newsletter and Protocol sections .