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EMS Technical Tips
Incubation Solution Additive AURION BSA-c™
Detailed information is provided in the package inserts.
Recommended Incubation Solution:
PBS,
(10 mM Phosphate buffer, 150 mM NaCl)
0.1-0.2 % AURION BSA-c™
15 mM NaN3
pH 7.4
After addition of the BSA-c™ concentrate the pH should be checked and adjusted to 7.4 if necessary.
This Incubation Solution is used throughout the whole set of reactions and washing steps (i.e. from immediately after the blocking step, through the primary antibody incubations, in-between washing steps, secondary incubation and the final washing steps).
Additional Information
"Optimized Immuno Labelling using AURION BSA-c™ and AURION Blocking Solutions" by J.L.M. Leunissen and P.F.E.M. van de Plas
For additional information please see the Aurion Immunogold Newsletter and Protocol sections