R-Gent Silver Enhancement Reagents Application Instructions

R-Gent SE-EM for Electron Microscopy

Post-embedding enhancement (on-grid)

Place grids on drops of the enhancement mixture arrayed on a sheet of Parafilm™.

Pre-embedding enhancement

Incubate specimens (e.g. vibratome sections) in Petri dishes or Eppendorf tubes containing the enhancement mixture, with agitation. When enhancement is completed, the specimens are washed with distilled water. A fixation step with photographic fixer is not required.

Storage

The AURION R-GENT SE-LM components are stored at 4°C and allowed to reach room temperature before use. SE-EM INITIATOR may be stored at -20°C for prolonged shelf life.

R-Gent SE-LM for Light Microscopy

- Slide or Coverslip Labeling

A few drops of the freshly prepared enhancement mixture are applied to cover the specimen. During enhancement the specimens are kept in a moist chamber. The ongoing enhancement may at intervals be monitored with an inverted light microscope. When enhancement is judged to be complete, the specimens are washed with distilled water. A fixation step with photographic fixer is not required.

- Whole Mount Labeling

Specimens like floating (vibratome) sections can be enhanced in Petri dishes or 6-24 well plates.

- Bio Assays

Depending on the type of assay the enhancement for bio assays can be performed in sealed plastic bags, Petri dishes or in disposable screw cap sealed tubes.

Additional Information

For additional information please see the Aurion Immunogold Newsletter and Protocol sections