EMS Technical Data Sheets

Technovit H7100 / H8100 Acid Phosphatase

EMS Catalog # 14653-14654

Acid Phosphatase For Glycol Methacrylate Sections


  1. Incubate sections in the incubating medium at 37°C for five to 12 hours. Long incubation periods are needed to get significantly visible reaction product.
  2. Wash in distilled water for two minutes.
  3. Counterstain with Methyl Green for five minutes.
  4. Wash in distilled water for two minutes.
  5. Air dry and cover slip.


Nuclei dark green
Cytoplasm light green
Sites of enzyme activity red


Incubating Medium:

Buffer Solution:

Do not adjust the pH of the buffer and store at 4°C.

Substrate solution:

Pararosaniline Solution:

Use heat to dissolve, filter when cool and store at 4°C

Sodium Nitrite Solution:

Prepare fresh and store at 4°C.
Methyl Green


Gerrits, P. O. and Smid, L., "Staining Procedures for Tissues Embedded in 2-Hydroxyethyl Methacrylate", Heraeus Kulzer.

Product Information

Technovit Glycol Methacrylate embedding kits H7100 / H8100