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EMS Technical Data Sheets
Giemsa Stain For Glycol Methacrylate Sections
EMS Catalog # 14653-14654
Technovit H7100 / H8100 Giemsa Stain
This Giemsa stain works well for a variety of tissues.
Procedure
- Stain in Giemsa working solution at room temperature for 1½ hours.
- Rinse in dilute acetic acid (four drops in 100 ml DI water) for two seconds.
- Dip in 96% alcohol.
- Dip in 96% alcohol.
- Rinse in isopropyl alcohol three times for two minutes each.
- Clear in xylene and mount.
Results
| Nuclei | violet |
| Cytoplasm | blue |
| Erythrocyten | pink |
Solutions
Giemsa Solution:
- 0.15 g Azure A
- 0.30 g Methylene Blue
- 0.36 g Eosin Y
- 0.04 g Phloxine B
- 50 ml Glycerine
- 40 ml Methanol
- Add dyes to Glycerine and methanol, mix well. Incubate overnight in 55-58°C oven stirring occasionally. Stable for year.
Giemsa Working Solution:
- 4 ml Giemsa stock solution
- 40 ml phosphate buffer solution
Phosphate Buffer Solution:
- 7.25 g Sodium phosphate, monobasic
- 2.75 g Sodium phosphate, dibasic
- 1000 ml Distilled water
- 10 ml 10% Triton X-100
- Store at 4°C.