Poly-L-Lysine solution is intended for use as an adhesive subbing solution for immunoperoxidase and routine Histologic staining preparations.
The loss of paraffin and frozen sections from slides has long been a problem during routine Histologic staining procedures. Various adhesives including albumin, gelatin and chrome alum have been applied to slides to minimize this loss. 1-3 different solutions of Poly-L-Lysine have been shown to be most effective in promoting adhesive of sections. 4-5 The polycationic nature of this molecule allows interaction with the anionic sites of tissue sections resulting in strong adhesive properties. 5. Poly-L-Lysine has been demonstrated as an effective tissue adhesive for use in various microwave procedures.
Poly-L-Lysine Solution. Poly-L-Lysine, 0.1% (w/v), in deionized water. Preservative added.
Poly-L-Lysine solution is for "In Vitro Diagnostic Use". Normal precautions exercised in handling laboratory reagents should be followed. Observe all local, state and Federal laws when disposing of waste. Refer to Material Safety Data Sheet for any updated risk, hazard or safety information. When diluted Poly-L-Lysine solution is prepared according to instructions, the maximum number of slides that can be coated is 900 per liter of diluted solution. Exceeding 900 slides per liter will affect the performance of the product.
Dilute Poly-L-Lysine solution 1:10 with deionized water prior to coating slides. Use plastic containers and graduated cylinders when mixing or storing solution and coating slides. Do not add fresh solution to used diluted solution.
Dilute Poly-L-Lysine solution at room temperature (18-26°C). Reagent is stable until expiration date shown on label. Store diluted Poly-L-Lysine solution in refrigerator (2-8°C). The diluted solution is stable for at least three months. Filter diluted solution after use.
Discard solutions if turbidity or bacterial growth develops.
Material required but not provided: Microscope slides, slide rack, plastic containers & graduated cylinder, drying oven (optional)