Taylor's Method for Bacteria

EMS Catalog #: 26108-Series

Fixation:

10% Buffered Neutral Formalin (#15740)

Section:

Paraffin @ 6 microns

Staining Procedure:

Use Control Slide

1. Deparaffinize and hydrate to distilled water.
2. Harris Hematoxylin, (#26108-01) for 5-10 minutes.  Wash in running distilled water 1 minute.
3. Differentiate in Acid Alcohol, (#26108-06).  Wash in running water 3 minutes.
4. Wash in Lithium Carbonate Solution, (#26108-05) to intensify blue  ***From this point carry only one slide at a time.
5. Wash in running water, 5 minutes.  Hucker's Crystal Violet, (#26108-02) two minutes.  Wash quickly in water.
6. Mordant in Gram's Iodine, (#26108-04) 1 minute.  Wash in water.  Blot with filter paper moistened with water, but do not allow to dry.
7. Decolorize in Acetone:Alcohol, (#26108-07) until no more blue comes off.  Blot with filter paper moistened with Acetone:Alcohol, but do not allow to dry.
8. Basic Fuchsin Working, (#26108-3A) or (5.0ml Basic Fuchsin Stock (#26108-03) and 60.0ml Distilled water) for 3 minutes.  Wash in water.  Blot with filter paper moistened with water, but do not allow to dry.
9. Dip in Acetone, (#26108-08) until section begins to decolorize.
10. Differentiate in Picric Acid-Acetone, (A-108-9) until section becomes reddish-brown-yellow (15 seconds).
11. Pass quickly through Acetone-Xylene, 1:2, (#26108-10), then Acetone-Xylene, 1:3 (#26108-11).
12. Clear in Xylene, (#23400) two changes.  Mount.

Results:

References:

AFIP Manual of Histological Staining Methods, 3rd ed., Ed. L. Luna:  New York:  McGraw Hill Publications, c. 1968, p. 226.

Clark, G.:  Staining Procedures, Williams and Wilkins Company, Baltimore, 3rd Ed., c. 1973, p. 320

Taylor, R.D., Amer. J. Clin. Path., 46:472 (1966).

Product Information

Taylor's Method for Bacteria