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EMS Technical Data Sheets
Giemsa Thick Film Stain
EMS Catalog #: 26154-Series
Fixation:
None. Films are prepared by spreading 3 – 5 drops of blood on a scrupulously clean slide to form a circle of about 15 mm diameter: the films are air dried by keeping in a horizontal position for 18 – 24 hours at room temperature, protected from dust and insects.
Staining:
- Stain 40-120 minutes in Giemsa Thick Film Staining Solution (#26154-01). *
- To prepare Giemsa Thick Film Staining Solution from Giemsa Stock Solution (#26154-03), mix before use:
Giemsa Stock Solution (#26154-03) - 1 ml
Phosphate Buffer (#26154-02) - 2 ml
Distilled Water - 47 ml
- To prepare Giemsa Thick Film Staining Solution from Giemsa Stock Solution (#26154-03), mix before use:
- Wash 5-10 minutes in distilled water or Phosphate Buffer (#26154-02)
- Dry in the air and examine.
Stain Results:
| Malarial Parasites | Clear Red Chromatin |
| Cytoplasm | Clear Blue |
| Red Corpuscles | Not seen due to lacking of hemoglobin during drying |
References:
Clark, G., Staining Procedures, Williams and Wilkins Co. Baltimore, 3rd, c. 1973, p. 126
Lillie, R. D. Histologic Technic and Practical Histochemistry, 3rd ed., Mcgraw-Hill, New York, 1965.
Barber, M.A., and Komp, H.W., Pub. Health Rep., 44:2330-41, 1929