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EMS Technical Data Sheets
Hall’s Method for Bilirubin
EMS Catalog #: 26611-Series
Fixation:
10% Buffered Neutral Formalin (#15740).
Sections:
Paraffin @ 6 microns
Staining Procedures:
- Deparaffinize and hydrate to distilled water.
- Place in Fouchet’s Reagent (#26611-01) for 5 minutes. Wash in running water, then in distilled water.
- tain for 5 minutes in Van Gieson’s Solution (#26611-02)
- Dehydrate in 95% Alcohol, absolute alcohol, and clear in xylene, two changes each.
- Mount with Permount (#17986-01)
Stain Results:
| Biliverdin | Greeen |
| Collagen | Red |
| Muscle | Yellow |
References:
Hall, M.J., Amer. J. Clin. Path., 34:313-316, 1960.
Luna, L.G. (ed). Manual of Histologic Staining Methods of the Armed Forces Institute of Pathology, 3rd ed., McGraw-Hill, N.Y., c. 1968, p.174.