Liquid Release Agent

EMS Catalog #70880

Flat-Embedding Method Using Liquid Release Agent

Electron Microscopy Sciences introduces Liquid Release Agent, a hydrosoluble mold-release agent for use in the flat embedding of microorganisms, and as a replacement for the "PTFE" coating of glass slides to produce a smoother, even surface. A routine, flat-embedding method using Liquid Release Agent is described by Reymond and Pickett-Heaps (1983).

This article describes the selection and precisely oriented sectioning of single cells by light microscopy, prior to examination by electron microscopy.

Preparation Of The Microscope Slide:

Microscope slides are dipped in the Liquid Release Agent to the level of the frosted glass, withdrawn, and dried in a dust-free environment for two hours at room temperature. (An EMS DIP MISER - Catalog #70510 can be used to economize on the Release Agent.) The slides then are put into a slide box or a staining dish, and dried overnight at 40 o - 70°C. At this point the glass slides are ready for the procedure described by Reymond & Pickett-Heaps.

Note:

Incomplete drying of the release agent may prevent subsequent polymerization of the liquid epoxy resin.

EMS Products - Used in Reymond & Pickett-Heaps procedure:

• Liquid Release Agent Cat # 70880
• EMS Dip miser Cat # 70510
• EMS Tissue Adhesive Cat # 72590
• Frosted Glass Slides Cat # 71870

References:

Reymond, O.L. & Pickett-Heaps, J.D. (1983) Journal of Microscopy, Vol. 130, Part 1, p. 79

Online Ordering

Liquid Release Agent, EMS Dip miser, EMS Tissue Adhesive, and Frosted Glass Slides are available online from the EMS Catalog. For ordering or product information, on the product name.