The instrument consists of an OUTER TRANSPARENT CASE and an INNER SLIDE on which is printed a series of SCALES.
There are FOUR SCALE LINES for producing SCALE MARKS of 10µm, 1µm, 0.1µm (100µm) and 10nm (100A) respecitively. Each of these scale lines can be used for the production of other sizes of scale marks (see below).
The figures on each of the scale lines represent the PHOTOMICROGRAPH MAGNIFICATION IN THOUSANDS. For example: on the u\10µm scale line the figure 2 represents x2,000 and the figure .7 represents x700; on the .1µm scale line the figure 200 represents x200,000 etc...
Along the top edge of the SLIDE is a standard MILLIMETER SCALE.
To apply a 10µm scale mark to a photomicrograph of x 3,000:
Each of the four scale lines can be used for the production of any desired size of scale mark.
For example, to apply a 5µm scale mark, use the 10µm scale line and set the instrument at HALF the photomicrograph magnification. To apply a 0.5µm scale mark, use the 1µm scale line and set the instrument at HALF the photomicrograph magnification.
Desired scale mark/scale line used X magnification of photomicrograph
You wish to apply a 5µm scale mark to a photomicrograph of x 10,000.
Using the 10µm scale line the calculation reads:
5/10 X 10,000 = 5,000
Set the slide at x 5,000 on the 10µm scale line and the instrument is set to produce a 5µm scale mark.
You wish to apply a 25µm scale mark to a photomicrograph of x 600.
Using the 1µm scale line the calculation reads:
25/1 X 600 = 15,000
Set the slide at x 15,000 on the 1µm scale line and the instrument is set to produce a 25µm scale mark.
When the instrument is being used frequently for the application of scale marks to photomicrographs of less than x1,000, a 100µm scale mark may be the one most often required.
For this purpose, the 10µm scale line can be used as a 100µm scale line by considering the figures on the scale to represent the PHOTOMICROGRAPH MAGNIFICATION IN HUNDREDS (Instead of thousands). For example, the figure "6" would represent x 600 and the figure ".2" would represent x20.
For measuring the real-life size of structures from photomicrograph prints or negatives, the instrument is used in the same fashion as calipers.
Depending on the unit of measurement desired, different scale lines are used in the following procedure:
What is the real-=life size, in µm, of a structure which measures 22mm in a photomicrograph of x75,000?
In this case the desired unit of measurement is µm, therefore a reading is taken on the 1um scale line.
What is the real-life size, in the nm of a structure which measures 2cm in a photomicrograph of x1000,000?
What is the real-life size, in µm, of a structure which measures 4cm in a photomicrograph of x600?
µm = micrometer; nm = nanometer; Å = Angstrom Units
1 mm = 1,000µm; 1µm = 10,000A; 1nm = 10Å
To clean the surface use an Ammonia-Based glass cleaning solution or Warm Water and Detergent. Polish with a soft cloth or tissue.
NEVER attempt to remove ink, or other stains, with a solvent such as acetone which may damage the surface.
When not in use always store the instrument in the protective pouch. Do not expose in extreme heat or leave in direct sunlight.
Eventually the outer case of the instrument may become scratched. Although this will not affect the smooth operation of the instrument, the case may be polished using any good quality vinyl polish.
Photomicrograph Scale Marker™s are available online from the EMS Catalog. For ordering or product information, click here.