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Detailed information is provided on the package inserts.
In order to obtain a positive reaction when using these tracers the following should be kept in mind:
Anionic tracers are negatively charged at pH >5
Cationic tracers are positively charged at pH <10
BSA tracers are intended for tracking fine capillaries or connecting spaces in intact tissues.
For additional information please see the Aurion Immunogold Newsletter and Protocol sections