|16% Paraformaldehyde Solution||20 ml|
|50% Glutaraldehyde EM Grade||8 ml|
|0.2M Sodium Phosphate Buffer||25 ml|
|Distilled Water||25 ml|
Final mixture is 78 ml with 5% Glutaraldehyde, 4% Formaldehyde in 0.064M buffer. This mixture is extremely hypertonic, with an osmolarity of more than 2000m OSM .
A lower concentraction of Glutaraldehyde and Formaldehyde especially used for vascular profusion.
|16% Paraformaldehyde Solution||13 ml|
|50% Glutaraldehyde EM Grade||5 ml|
|0.2M Sodium Phosphate Buffer||50 ml|
|Distilled Water||32 ml|
Final mixture is 2% Paraformaldehyde, 2.5% Glutaraldehyde and 0.1M Buffer. If necessary, the osmolarity can be adjusted by adding sucrose to NaCl. The pH may also need to be adjusted to your desirable point. This mixture is the most effective general fixative for both plant and animal specimens.
Paraformaldehyde - Glutaraldehyde Solution (Karnovsky's Fixative)