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Fluoroshield with strong antifading agent, 1,4-phenylenediamine (PPD) is an aqueous mounting medium for preserving fluorescence of tissue and cell smears. This unique formula prevents rapid photobleaching of FITC, Texas Red, AMCA, Alexa fluoro 488, Alexa fluoro 594, tetramethyly rhodamine, and Redox. The fluorescence is retained during prolonged storage at 4°C in the dark. This medium contains phenylenediamine and is not suitable for immunofluorescence of Cy dyes, GFP, mCherry, Phycoerythrin (R-PE), phyocyanin (PC), and allophycocyanin (APC) and other fluorochromes proteins.
1.366 ± 0.002 (This number applies to this mounting medium in solution. Refractive indexes change when the water solvent evaporates and mounting media dries on slides. We do not have the means to measure the refractive indexes of dry mounting mediums; however we expect the numbers to go higher when dried. The refractive index of water is 1.3330)
May encounter problems with frozen brain or other frozen tissues with lots of fat.
Immunofluorescence, confocal microscopy.
Ready to use mounting medium with dark coffee color. This color does not interfere with Immunofluorescence.
2-8°C is recommended. Protect from light, DO NOT FREEZE.
Coverslip can be removed before sealing the edges. Soak slide in warm (37°C) distilled or deionized water for several minutes. Carefully and slowly move the coverslip. Soak in water for an additional few minutes to remove coverslip. Rinse slide several times with warm water to remove all mounting medium. The slide can be remounted again.
For research use only; not for use in diagnostic procedures. FOR IN VITRO LABORATORY USE ONLY
“In vitro laboratory products for research”