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EMS Technical Data Sheets
UAR - EMS Stain
EMS Catalog #22405
Description
EMS’ UAR-EMS Stain is a new negative stain which may be used as an alternative to Uranyl Acetate (cat. 22400).
Procedure
The UAR Stain is supplied as a concentrate. Please use the following dilutions:
- For most samples, including but not limited to, virus', dilute by 4x in distilled water
- If you are working with Bacillus, then a dilution of 15-40x is needed.
Staining will take place in 30 minutes at room temperature. For increased contrast of the stain, simply adjust the staining time.
Reference Article:
Nakakoshi, Masamichi, Hideo Nishioka and Eisaku Katayama, 2011. New versatile reagents for biological transmission electron microscopy that substitute for uranyl acetate. Journal of Electron Microscopy, 60(6): 401-407.