EMS Technical Data Sheets

Giemsa Thin Film Stain

EMS Catalog #: 26153-Series


Streak a drop of blood, leukocytes, rickettsiae culture, pus or other exudates over a sterile glass slide by means of another slide or cover glass. Fix film in Methyl Alcohol for 2-3 minutes.


  1. Place in Ethyl or Methyl Alcohol for 5 – 7 minutes. Air dry.
  2. Stain in the Giemsa Thin Film Staining solution (#26153-01)*. Because different cells accept the stain at varying rates, prepare 3 – 4 sample slides and stain from 15 – 20 minutes. Some membranous structures (Trypanosomes) may require 1 – 2 hour periods.
    1. To prepare Giemsa Thin Film Staining solution from Giemsa Stock solution (#26153-03), mix before use:
      Giemsa Stock Solution (#26153-03) - 1 ml
      Phosphate Buffer (#26153-02) -50 ml
  3. Wash in distilled water and air dry.

Stain Results:

Nuclei, additional Leukocyte features are similar to Wright Stain results. Reddish Purple
Plasmodic cytoplasm Blue
Chromatin Red


Clark, G., Staining Procedures, Williams and Wilkins Co. Baltimore, 3rd, c. 1973, p. 125

Product Information

Giemsa Thin Film Stain