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EMS Technical Data Sheets
Giemsa Thin Film Stain
EMS Catalog #: 26153-Series
Fixation:
Streak a drop of blood, leukocytes, rickettsiae culture, pus or other exudates over a sterile glass slide by means of another slide or cover glass. Fix film in Methyl Alcohol for 2-3 minutes.
Staining:
- Place in Ethyl or Methyl Alcohol for 5 – 7 minutes. Air dry.
- Stain in the Giemsa Thin Film Staining solution (#26153-01)*. Because different cells accept the stain at varying rates, prepare 3 – 4 sample slides and stain from 15 – 20 minutes. Some membranous structures (Trypanosomes) may require 1 – 2 hour periods.
- To prepare Giemsa Thin Film Staining solution from Giemsa Stock solution (#26153-03), mix before use:
Giemsa Stock Solution (#26153-03) - 1 ml
Phosphate Buffer (#26153-02) -50 ml
- To prepare Giemsa Thin Film Staining solution from Giemsa Stock solution (#26153-03), mix before use:
- Wash in distilled water and air dry.
Stain Results:
| Nuclei, additional Leukocyte features are similar to Wright Stain results. | Reddish Purple |
| Plasmodic cytoplasm | Blue |
| Chromatin | Red |
References:
Clark, G., Staining Procedures, Williams and Wilkins Co. Baltimore, 3rd, c. 1973, p. 125