Prussian Blue Method for Hemosiderin
EMS Catalog #: 26170-Series
Fixation:
10% Buffered Neutral Formalin (#15740-01).
Sections:
Paraffin
Staining:
- Deparaffinize and hydrate to Distilled Water.
- Place sections in working staining solution for 30 minutes) at 60°C or for one hour at room temperature. Rinse in distilled water.
- Prepare working stain by mixing equal volumes of Potassium Ferrocyanide, 2% (#26170-01) and Hydrochloric Acid, 2% (#26170-02). Prepare fresh just before use.
- Counterstain in Safranin O, 0.2% (#26170-03) for 2 minutes. Wash in Acetic Acid 1% (#26170-04)
- Dehydrate in 95% Alcohol, Absolute Alcohol, clear in Xylene , and mount in synthetic resin, such as DPX (#13510)
Stain Results:
Hemosiderin |
Blue or Green |
Nuclei |
Red |
Background |
Pink |
References:
Perls, M., Virchow’s Arch. F. Path. Anat., 39:42-48, 1867.
Highman, B., Arch. Path., 33:937-938, 1942.
Lillie, R.D., Histopathologic Technic and Pratical Histochemistry, 3rd. ed., McGraw Hill, N.Y., 1965
Clark, G., (ed.), Staining Procedures, 3rd ed., Williams & Wilkins, Baltimore, p. 157, c. 1973
Product Information
Prussian Blue Method for Hemosiderin