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Alcoholic Formalin
Paraffin, Ester Wax, Celloidin or Gelatin
Bring sections to Alcohol, 50% (#26206-02) and rinse rapidly in distilled water.
Cover sections with Alizarin Red S (#26206-01) and follow the staining by transmitted light, on the stage of a staining microscope. Calcium sites will be covered with a vivid orange-red calcium-alizarin lake within a period of 30 seconds to 5 minutes. The end point should be chosen when the deposit is heavy but not too diffused.
Calcium Sites | Covered and surrounded by heavy orange red precipitate which is birefringent between crossed polaroids |
Background | Faint Pink |
McGee-Russell, S.M., J. Histochem., 6:22-42, 1958.
Clark, G., (ed.). Staining Procedures, 3rd ed., Williams & Wilkins, Baltimore, p. 140, c. 1973.