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Buffered Neutral Formalin, 10%
Paraffin @ 6 microns
Calcium oxalate | Black |
All other tissue elements | Red |
Note: If a large amount of egg albumin section adhesive is present, numerous gas bubbles will develop during the light bulb staining treatment (step 2). This tends to inhibit the reaction. Should this occur, pour off the mixture and add fresh solution.
Pizzolato, P., J. Histochem. Cytochem., 12:333-336, 1964.
Luna, L.G., (ed), Manual of Staining Methods of the AFIP, 3 rd edition, McGraw-Hill, NY, c 1968, p 177.
Silver Nitrate, 5% Aqueous
Hydrogen Peroxide, 30%
Nuclear Fast Red (Kennechtrot) Solution
Pizzolato's Method For Calcium Oxalate