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EMS Technical Data Sheets
Maximow's Method for Bone Marrow
EMS Catalog #: 26252-Series
Fixation:
Zenker’s / Formalin
Sections:
Paraffin at 6 microns
Staining Procedures:
- Deparaffinize and hydrate to distilled water.
- Mayer’s Hematoxylin (#26252-01) for 15 minutes. Wash in tepid tap water for 15 minutes.
- Stain in working solution overnight. Prepare working solution fresh each time as follows:
- Eosin Y, 0.1%, Aqueous (#26252-02) - 10 ml
Triple distilled water or Phosphate Buffer (#26252-04) - 100 ml
Azur II, 0.1%, Aqueous (#26252-03) - 10 ml
Glacial Acetic Acid - 2 drops
- Eosin Y, 0.1%, Aqueous (#26252-02) - 10 ml
- Differentiate in 95% alcohol until blue ceases to come out into alcohol and erythrocytes and collagen are pink.
- Dehydrate in absolute alcohol, clear in xylene, two changes each.
- Mount with Permount (#17986-01).
Stain Results:
| Nuclei | Blue |
| Basophile leucocyte, mast cell | Purple to Violet |
| Cartilage | Purple |
| Erythrocytes, eosinophile granules | Pink |
| Cytoplasm | Blue to Pink |
References:
AFIP, Manual of Histologic Staining Methods, 3rd edition, Ed. L. Luna: New York: McGraw Hill Publications, c. 1968, p. 120
Mallory, F.B., Pathological Technique, New York, Hafner Publishing Co., 1961, p. 196.