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EMS Technical Data Sheets
Crystal Violet Stain For Amyloid
EMS Catalog #26301
Fixation
Absolute alcohol or 10% Formalin
Sections
Paraffin
Staining
- Stain sections of fixed or unfixed tissue in Crystal Violet Solution for three to five minutes.
- Wash in Acetic Acid, 10%, followed by a thorough washing in water to remove all traces of acid.
- Examine in water or glycerine. The stain will keep for some time if sections are mounted in a saturated solution of potassium acetate or in levulose.
- Mount in Modified Apathy's Gum Syrup or Lieb's Abopon.
Stain Results
| Amyloid | Violet-Red |
| Tissue | Blue |
References
Clark, G.: Staining Procedures, Williams and Wilkins Co., Baltimore 3 rd . ed., c. 1973,p.48.
Conn , H.J., Biological Stains 4 th ed. Biotech Publications, Geneva New York , 1940.