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EMS Technical Data Sheets
Mayer's Mucihematein, Modified
EMS Catalog #: 26321-Series
Fixation:
Absolute Alcohol (Cat. #15055) 4 – 8 hours.
Sections:
Paraffin or Celloidin.
Staining Procedures:
- Deparaffinize and hydrate to distilled water.
- Place on staining rack and rinse several times with distilled water.
- Place 2 ml of Mayer Mucihematein, Modified (#26321-01) on each slide. Allow to remain for five minutes. Drain off stain and wash well with distilled water three times for 5 minutes each.
- Dehydrate in two changes of 95% alcohol, two changes of absolute alcohol, Absolute Alcohol-Xylene (50:50), and two changes of Xylene (#23400).
- Mount with Permount (#17986-01) or DPX (#13510).
Stain Results:
| Mucin | Deep Violet |
| Cell Nuclei | Pale Gray-Blue |
| Connective Tissue | Pale Gray to Colorless |
References:
Laskey, Alice: Stain Techn., 25:33 (1950).
Clark G.: Staining Procedures, William & Wilkins Company, Baltimore, 3rd Ed., c. 1973, p. 47