Lillie Modification of Mason's Trichrome Stain
EMS Catalog #: 26364-Series
Fixation:
Bouin’s (#15990-01) or Russell’s Zenker -contain no Mercury (#64123-05).
Sections:
Paraffin at 6µm.
Staining Procedures:
- Deparaffinize and hydrate to distilled water.
- If Zenker’s contain Mercury is used as fixative, remove Mercuric Chloride Crystal by placing in Lugol’s Iodine (#26364-07) or Gram’s Iodine (#26364-7A) for 15 minutes. Rinse in water and clear in Sodium Thiosulfate, 5% (#26364-08) for 3 minutes. Wash in water for 10 minutes or longer. If Russell’s Zenker (#64123-05) is used, go to step #3.
- Stain in Weigert’s Iron Hematoxylin Working* solution for 5 minutes. Wash in running water.
- * To prepare working solution, mix equal parts of Weigert’s Iron Hematoxylin A (#26364-02) and Weigert’s Iron Hematoxylin B (#26364-03).
- Stain in Biebrich Scarlet Solution (#26364-04) for 4 minutes. Rinse in water
- Mordant in Phosphomolybdic Acid-Phosphotungstic Acid (#26364-01) for 1 minute.
- Stain in Fast Green FCF Solution (#26364-05) for 4 minutes.
- Differentiate in Acetic Acid, 1% (#26364-06) for 1 minute.
- Carry through alcohol and dehydrate with acetone or absolute alcohol.
- Clear and mount in balsam saturated with salicylic acid.
Stain Results
Nuclei |
Black |
Cytoplasm |
Brown to Pink |
Muscle |
Red |
Erythrocytes |
Brilliant Scarlet |
Collagen |
Blue to Blue-Green |
Myelinated Fibers |
Red |
Fibrin |
Red to Pink |
References:
Clark, G.: Staining Procedures. Williams and Wilkins Co., Baltimore, 3rd ed., c. 1973, p. 59
Lillie, R.D., Arch. Path., 29:207, 1940.
Product Information
Lillie Modification of Mason's Trichrome Stain