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EMS Technical Data Sheets
A Modified Verhoeff Elastic-Van Gieson Stain
EMS Catalog #26369
Consistent and shorter staining time!
Fixation
10% Buffered Neutral Formalin
Sections
4-5 microns
Staining
- Deparaffinize and hydrate sections.
- Stain in modified Verhoeff at room temperature for 7 minutes or in a 60 °C oven for 3 1/2 minutes.
To prepare modified Verhoeff Working Elastic solution, mix just before use:
• 3 parts Alcoholic Hematoxylin, 3%
• 2 parts Ferric Chloride 2%, aqueous
• 1 part Lugol's Iodine - Wash in running warm tap water for 1 minute.
- Differentiate in Ferric Chloride, 0.4% aq. for 75 seconds.
- Wash in running warm tap water for 5 minutes.
- Counterstain in Van Geisons solution for 60 seconds.
- Dehydrate, clear and mount with Permount.
Results
| Nuclei | Greyish-black |
| Elastic Fibers | Bluish-black |
| Collagen fibers | Red |
| Muscle | Greenish yellow |
References
Garvey, Winsome et al "A modified Verhoff Elastic Van Geison Stain" The Journal of Histotechnology, Vol. 14, No. 2 pp113-114 June 1991.
Product Information
Alcoholic Hematoxylin, Ferric Chloride 2% aqueous
Lugol’s Iodine
Ferric Chloride 0.4% aqueous
Van Geisons Solution
Modified Verhoeff Elastic-Van Gieson Stain