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10% Buffered Neutral Formalin or Bouin's Solution
Note: If formalin fixative is used, decerated tissue sections must be mordanted in Bouins Fluid for 1 hour in a 50 °C oven. Wash well in running water to remove picric acid deposits.
4-6 microns
Orcein | 0.2% 25.0 ml |
Alcoholic Hematoxylin | 5% 8.0 ml |
Ferric Chloride | 10% 5.0 ml |
Lugol's Iodine | 5.0 ml |
Nuclei | Black |
Cytoplasm | Red |
Elastic Fibers | Purple to Black |
Collagen and Bone | Yellow |
Mucopolysaccharides | Blue-Green |
Muscle | Red |
Movat, H.Z .,: Demonstation of all C onnecti ve Tissue Elements in a Single S ection.
Arch Path., 60:289-295, 1955.
Roman N., et al: Orcein-Hematoxylin in I odize d Ferric Chloride as a Stain for E lastic T issue.
Stain Techn ., 42:199-202, 1967.
Silverman Jefferey., A Modified Movat Pentachrome Stain. Histologic , p. 33. 1972
Alcian Blue 1%
Alkaline Alcohol
Orcein 0.2%
Hematoxylin Alcoholic 5%
Ferric Chloride 10%
Lugol’s Iodine
Woodstain Scarlet-Acid Fuchsin Working Solution
Acetic Acid 0.5%
Phosphotungstic Acid 5%
Alcoholic Saffron
Modified Movat's Stain