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EMS Technical Data Sheets
Toren's Method For Mast Cells
EMS Catalog #26472
Fixation
10% Buffered Neutral Formalin
Section
Paraffin, @ 6 Microns
Staining Procedure
- Deparaffinize and hydrate to distilled water.
- Stain in *Working Giemsa Solution for 75 minutes. Rinse in distilled water.
***Prepare Working Giemsa Solution by adding 40 drops of
Giemsa Stock Solution, to 40 ml of distilled water and mixing thoroughly.
Prepare fresh, immediately before use. - Place in Acetic Acid, 0.5%, for 5 to 10 seconds. Rinse in distilled water.
- Stain in Pollack's Trichrome Solution, for 10 to 15 seconds.
- Rinse in Acetic Acid, 0.5%, until no more stain washes off, 3 to 5 seconds.
- Dehydrate in 95% Alcohol, Absolute Alcohol and clear in Xylene.
- Mount with Permount.
Results
| Mast Cell Granules | deep blue |
| Collagen & Cartilage | blue green |
| Muscle & Elastic Fibers | red |
| Fibrin | purple |
| Bone | green |
| Colloid, Keratin & Erythrocytes | orange red to orange |
References
Toren, D.A.: Stain Techn ., 38:249-250, 1963.
Luna, L., (ed.), Manual of Histologic Staining Methods of the AFIP , 3 rd . ed.,
McGraw-Hill , New York , c. 1968, pp. 116-117.
Product Information
Giemsa Stain Solution
Acetic Acid 0.5% Aqueous
Pollacks’s Trichrome Solution
Toren's Method For Mast Cells