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EMS Technical Data Sheets
Gomoris’ Methenamine Silver for Urate Crystals
EMS Catalog #: 26614-Series
Fixation:
Absolute Alcohol at 4°C for overnight.
Sections:
Paraffin @ 4 - 5 microns
Staining Procedures:
- Deparaffinize to Xylene and rinse with 3 changes of absolute alcohol.
- Place slides in preheated working* Methenamine Silver. Incubate for 30 minutes @60°C.
To prepare working solution:
Methenamine-Silver Nitrate Stock Solution:
Silver Nitrate, 5% (#26614-01) - 5 ml
Methenamine, 3% (#26614-02) - 100 ml
Methenamine-Silver Nitrate Working Solution:
Methenamine Silver-Nitrate Stock Solution: - 25 ml
Distilled water: - 25 ml
Sodium Borate, 5% (#26614-03): - 2 ml
Prepare fresh prior to use. DO NOT USE IF CLOUDY. Urate Crystals should be black
- Rinse sections in distilled water.
- Tone with Gold Chloride, 0.1% (#26614-04) for 5 minutes.
- Give sections 4 or 5 rinses of distilled water.
- Sodium Thiosulfate, 3% (#26614-05) for 5 minutes.
- Wash in tap water for 5 minutes.
- Rinse in distilled water.
- Counterstain in Light Green Working Solution (#26614-06) for 2 minutes.
- Dehydrate, clear and mount with Permount (#17986-01) or any synthetic resin
Stain Results:
| Urate Crystals | Black |
| Background | Green |
References:
Sheehan, Denza C. and Hrapchak, Barbara B. Theory and Practice of Histotechnology, 2nd ed. The C.V. Mosby Company, St. Louis, p.225 – 226, c. 1980.