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EMS Technical Data Sheets
Woelck’s Method for Myelin Sheath
EMS Catalog #: 26684-Series
Fixation:
Formalin 10% Neutral Buffered (#15740).
Sections:
Paraffin @ 15 microns.
Staining Procedures:
- Deparaffinize and hydrate to distilled water.
- Mordant in Ferric Ammonium Sulfate, 2.5% (#26684-01) overnight. Rinse in two changes of distilled water.
- Working Hematoxylin solution for 2 hours. To prepare working solution mix the following ingredients in order listed just before use:
45.0 ml Distilled water
10.0 ml Hematoxylin Stock (#26684-02) – filtered
7.0 ml Lithinum Carbonate, Saturated (#26684-03) – filtered
45.0 ml Distilled water - Rinse in two changes of distilled water.
- Differentiate in 80% Alcohol until background is clear and myelin stands out sharply.
- Dehydrate with 95% Alcohol, absolute alcohol, and clear in Xylene (#23400) two changes each. Mount.
Stain Results:
| Glial Cells, Nucleoli of Neurons | Black |
| Myelin Sheath | Blue |
| Background | Clear |
References:
AFIP, Manual of Histological Staining Methods, 3rd ed., Ed. L. Luna: New York: McGraw-Hill Publications. C. 1968, p. 206.