Questions? 800-523-5874 | [email protected]
- Prepmaster™ Specimen Preparation Robot
- TEM Grids
- TEM Window Grids
- Omniprobe Nanomanipulation Systems
- K-kit Wet "Liquid" TEM Kit
- Specimen Mounts
- SEM Specimen Holders
- Index and Finder SEM Grids
- SEM for Forensics
- SEM Sample Preparation Station Materials
- Cryogenic Personal Protection Equipment
- Cryo Dewars & Flasks
- Cryogenic Grids & Accessories
- Cryogenic Vials & Racks
- Cooling Chambers & Ice Baths
- Prepmaster™ Specimen Preparation Robot
- Laboratory Microwave Ovens
- LYNX II Automated Tissue Processor
- EMS Poly III
- Microtomes
- Tissue Slicers
- Rapid Immersion Freezer
- Heaters & Chillers
- SEM Cooling Stage
- Glow Discharge Systems
- Sputter Coaters & Carbon Coaters
- Stages
- Freeze Dryers
- Critical Point Dryers
- Cryo-SEM Preparation System
- Specimen Transfer Systems
- Decontaminators
- Desiccators
- Centrifuges
- Dry Baths
- Stirrers, Hot Plates
- Vortexers & Magnetic Mixers
- Rotators & Rockers
- Ovens & Incubators
- Vibration Isolation
- Air Sampling
- Vacuum Pumps
EMS Technical Data Sheets
Ehrlich’s Hematoxylin
EMS Catalog #: 26753-Series
Fixation:
Formalin 10% Neutral Buffered (#15740) or fixative of choice
Sections:
Paraffin @ 5 -6 microns.
Staining Procedures:
- Deparaffinize and hydrate to distilled water.
- Stain for 2 – 5 minutes in Ehrlich’s Hematoxylin (#26753-01). Wash in distilled water.
- Differentiate in Acid Alcohol (#26753-02) until the sections turn red.
- Blue section in Sodium Acetate, 1% (#26753-03) or Ammonium Water (#26753-3A) or Lithium Carbonate Solution (#26753-3B)
- Wash thoroughly and counterstain.
References:
P. Ehrlich "Fragekasten" ["Questionbox"] Zeitschr. für Wiss. Mikr. Techn. III,I:150, 1886. Article in German.
Mallory, F.B., Pathological Technique. W.B. Saunders Co, Philadelphia, p. 73, 1938.
Clark, G., (ed). Staining Procedures, 3rd edition, Williams & Wilkins, Baltimore, pp 33-34, 1973.
Luna, L.G. (Ed), Manual of Histologic Staining Methods of the AFIP, 3rd Edition, McGraw-Hill, NY, pp 35 and 38, c. 1968.