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- Prepmaster™ Specimen Preparation Robot
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EMS Technical Data Sheets
Harris Hematoxylin (With or Without Mercury)
EMS Catalog #: 26754-Series
Fixation:
Formalin 10% Neutral Buffered (#15740) or fixative of choice
Sections:
Paraffin @ 5 -6 microns
Staining Procedures:
- Deparaffinize and hydrate to distilled water
-
- Stain in Harris Hematoxylin (#26754-01) containing 2 – 4 ml Glacial Acetic Acid per 100 ml for 5 – 15 minutes. Wash in water.
- If using Harris Hematoxylin w/o Mercury (#26754-1A) containing 2 – 4 ml Glacial Acetic Acid per 100 ml for 5 – 15 minutes. Wash in water.
- Differentiate in Acid Alcohol (#26754-03) until the sections turn red.
- Blue section in Sodium Acetate, 1% (#26754-04) or Ammonium Water (#26754-4A) or Lithium Carbonate Solution 26754-4B).
- Wash thoroughly and counterstain.
References:
Harris, H.F., J. Appl. Microscopy, III: 777-781, 1900.
Mallory, F.B., Pathological Technique. W.B. Saunders Co, Philadelphia, 1938.
Clark, G., (ed). Staining Procedures, 3rd edition, Williams & Wilkins, Baltimore, pp 33-35, 1973.
Luna, L.G. (Ed), Manual of Histologic Staining Methods of the AFIP, 3rd Edition, McGraw-Hill, NY, pp 34 and 38, c. 1968.