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EMS Technical Data Sheets
Weigert's Iron Hematoxylin With Metachromatic Dyes Fixation
EMS Catalog #26758
Formalin, Bouin, Carnoy, Orth's or Zenker's
Sections
Paraffin
Staining
- Deparaffinize and hydrate to distilled water.
- Stain in working Weigert's Solution* for 6 minutes. Wash in water. *Prepare working solution by mixing equal volumes of Weigert's Iron Hematoxylin A and Weigert's Iron Hematoxylin B.
- Stain in 0.02% Fast Green FCF for 3 minutes.
- Wash in 1% Acetic Acid.
- Stain for 4-6 minutes in 0.1% Bismarck Brown or in 0.1% Safranin O.
- Dehydrate with 95% alcohol and 2 changes of absolute alcohol.
- Clear with Alcohol: Xylene, 1:1, 1 change and two changes of Xylene.
- Mount with a synthetic resin.
Staining Results
| Nuclei | Black |
| Cytoplasm | Gray-green |
| Mucus, cartilage and mast cell granules | Yellowish brown, deep red or orange -red depending on dye used. |
References
Weigert, K., Z. Wissen. Mikr., 21:1-5, 1904.
Lillie, R.D., Histopathologic Technic and Practical Histochemistry , 3 rd , edition, McGraw-Hill , NY , 1965.
Clark , G., (ed.), Staining Procedures , 3 rd edition, Williams & Wilkins, Baltimore , p. 40, c 1973.
Product Information
Weigert’s Iron Hematoxylin Sol A
Weigert’s Iron Hematoxylin Sol B
Fast Green FCF Solution 0.02%
Bismark Brown Y Solution 0.1%
Safranin O 0.1%
Acetic Acid 1% Aqueous
Weigert's Iron Hematoxylin with Metachromatic Dyes Fixation