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Formalin, Bouin, Carnoy, Orth's or Zenker's
Paraffin
Nuclei | Black |
Cytoplasm | Gray-green |
Mucus, cartilage and mast cell granules | Yellowish brown, deep red or orange -red depending on dye used. |
Weigert, K., Z. Wissen. Mikr., 21:1-5, 1904.
Lillie, R.D., Histopathologic Technic and Practical Histochemistry , 3 rd , edition, McGraw-Hill , NY , 1965.
Clark , G., (ed.), Staining Procedures , 3 rd edition, Williams & Wilkins, Baltimore , p. 40, c 1973.
Weigert’s Iron Hematoxylin Sol A
Weigert’s Iron Hematoxylin Sol B
Fast Green FCF Solution 0.02%
Bismark Brown Y Solution 0.1%
Safranin O 0.1%
Acetic Acid 1% Aqueous
Weigert's Iron Hematoxylin with Metachromatic Dyes Fixation