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10% Buffered Neutral Formalin (#15741-01).
Paraffin @ 6 microns.
Nuclear Chromatin | Deep Red-Purple |
Cytoplasm | Light Green |
The Chromatin of plasmodia, sarcosporidia toxoplasma and histoplasma | Pale Red |
NOTE: If the fast Green is too intense, it may be removed promptly in water, it does not wash out in alcohol.
Lillie, R.D., Histopathologic Technic and Practical Histochemistry, 3rd ed, McGraw-Hill. NY, pp 149-150, 1965.
Luna, L.G., (ed). Manual of Histologic Staining Methods of the AFIP 3rd edition. McGraw-Hill, NY, p. 131, c. 1968.
Lillie’s Method for Nucleic Acid