Paraffin tissue microarrays (TMAs) are becoming more common in the laboratories. Current techniques including organizing, punching, and inserting tissues is time consuming and involving costly equipments. This mold is the simple tool but great answer for this current technique for laboratories to perform TMAs with less time consuming, excellent results.
It can be used for paraffin and frozen tissue microarrays. Create your own OCT/paraffin - TMA block, then with a single dermal needle (sterile or non-sterile), punch your fresh frozen or paraffin tissue and insert into the block.
With this mold, you can process up to 150 specimens onto one single slide with very little time consuming, minimal technical training.
The Arraymold Kit F generates 3mm diameter sample core and 35 cores per mold.
Kit consists of:
The rubber mold is reusable, remaining flexible over many uses and also allows for:
Following these steps will help you set the punches in your paraffin block and create a flat surface for cutting.
(Note: If not using all the array, just fill the empty area with paraffin.) After punching is complete:
When not in use, store array mold in its box, in a dark, dry place such as a drawer. Keep it away from chemicals and light.
Optional Temperature Conditions
The array mold is optimal at -10°C to -30°C. Do not snap freeze the mold in liquid nitrogen or other type of frozen chemical bath, as this will crack/damage it. Freezing the mold voids any warranty. The array mold is designed to use in a cryostat as well as for paraffin molding. To prolong the life of the mold, pull the embedding medium out carefully.
Recommended Paraffin for TMA Construction
Paraplast Xtra, Catalog #19214 (1kg) or Catalog #19215 (8kg) Note: The tissue may be processed in your usual paraffin, this is just for making the TMA blocks. Please test other paraffins for your desired applications before using.
Note: DOES NOT work with Richard Allen Type 3 and Type 5 paraffin.
Damage to the Array Mold
Minimal damage to the mold, such as losing a few rubber rods or cracks or splits in the mold may not necessarily make the mold totally unusable. Always be careful and gentle when pulling paraffin out of the mold. If the mold splits, it is sometimes possible to put a rubber band or tape it back together.
Dermal Needles/Biopsy Punches
All needle brands are slightly different in size. We recommend EMS catalog #6903-Series biopsy punches and stylers. Note: If experiencing difficulty when in pushing the stylet into the dermal needle, do this before using the needle: gently push and twist the stylet down the needle before beginning an array. Once the stylet is through the needle, push it in and out several times.
Using Cryo Array Punches
Be sure the punches are set into the embedding medium. Do this by melting the surface of the cryo array block on a metal plate and freeze it, again, before sectioning it in a cryostat. This step may need to be repeated if going deeper into the cryo array block.
The 2mm 60 core array mold is 7mm. The 1.5mm 150 core is 4mm deep. The 1.5mm core depth is not as deep as the 2mm is that since the 1.5mm cores are thinner, the shortened depth cores strengthens the rods and they last longer. Judging core depth: Use a marking pen and mark the needle to the maximum depth and stay within this distance. This is especially important when punching with the 1.5mm array mold needle.