Poly-L-Lysine Coated German Glass Cover Slips
EMS Catalog #72292-01 through 72292-20
Important
Store in Freezer – Fragile Glass Inside
- Air shipped at room temperature
- Once received, store unopened bags at -20°C
- Expiration: 6 months at -20°C
Handling Procedure
- Take a package from freezer and cut a bag of 15 pieces off the package – cut along middle seal line so that both bags remain sealed after cut – then put package back into freezer.
- Spray bag of 15 pieces with 70% ethanol in hood and let it dry completely.
- Cut to open bag and pour all coverslips into a well of a 6-well plate. Cover it and vigorously shake the plate side to side (horizontally) to loosen coverslip.
- Invert the plate so that the coverslips sit on the lid of the plate.
- Let coverslips roll along edge of the lid to separate from each other. Pick them up easily with sharp forceps.
- Store unused coverslips at room temperature in TC hood and use them within 2 weeks. (If longer storage is desired, place one coverslip per well and store the plate at 4°C.
Optional: Pre-incubate coverslips in culture medium before seeding cells.
Removing Coverslip from Well after Culture
- Create a lifting tool by bending a needle 0.5-1 mm from tip.
- Use one hand with the lifting tool to lift coverslip from its edge and use other hand with forceps to hold and remove coverslip from well.
Note: Do not drop the coverslip and remember which side contains cultured cell.
Coating Compatibility
Different cells require different coating substrates. If this type of coating did not produce satisfactory results, try a different coating substrate.
Product Information
Poly-L-Lysine Coated German Glass Cover Slips