EMS Technical Data Sheets

UV Stable Adjustable Pipetters

EMS Catalog #72430 Series

IntroductionUV Stable Adjustable Pipetters

This air-displacement pipette is made from temperature and chemical-resistant materials. It features a digital volume meter with maximum volume for the pipette is shown on the plunger button. Also, the pipetter has a tip ejector for easy removal or the used tip.

How to Use


Volume Setting

Attach Tips

Aspirating Liquid

Dispensing Liquid


Special Considerations

To ensure accuracy, rinse new tips by aspirating and dispensing liquid three times before pipetting.


Test can be performed by weighing the volume using an analytical balance with a suitable level of sensitivity.

The distilled water, weighing vessel, pipette and pipette tip must all be the same temperature.

NOTE: Pipettes were tested during production in accordance with the test conditions for pipetting water samples. If the accuracy of the pipetted volume is in question, check to make sure that:
... The pipette is not leaking.
... The temperatures of the sample, the pipette and the air are the same.
... The sample does not have a different density than water.
... The pipetting speed is not too fast.

If these checks prove unsuccessful, it is safe to assume that the calibration has to be altered.

In Case of Error

From the technical standpoint, this is a zero-decimal shift. The value by which the setting of the pipetter is shifted remains constant across the entire measuring range.


Take a 10-100µl. Follow up alignment of 1µl takes place at 100µl (=1%), the pipetter is also adjusted by 1µl at 10µl (=10%).

  1. Pipette, tip and water must be the same temperature. (20-25°C), =/-0.5)
  2. With an adjustable pipette, the pipette is set to the nominal volume required.
  3. With pipette tip attached to the pipette, pipette and weigh the desired volume 10 times.
  4. The mean of this weighing is converted into µl using the following formula: Volume = Weight/Density of liquid (at the specified temperature) NOTE: To align volume displayed, insert wrench (Figure 1) horizontally into the alignment opening in the pipette grip. (When doing so, hold onto the pipette vertically.) To reduce volume, turn the setting ring clockwise; to increase the volume, turn the setting ring counterclockwise (Figures 2-4).

NOTE: When setting ring is rotated either way, the piston stroke of the pipette is altered, although the volume setting remains unchanged.

  1. Remove the wrench and move the setting ring backwards and forwards until the counter and stroke system lock together.
  2. Repeat step 3. The readings must be within the tolerances specified. If the nominal volume does not correspond with the measuring result, repeat step 4.

  3. NOTE:
    Since this adjustment affects the entire measuring range, it is imperative to check the other volumes of this pipette specified in the technical data.

Varying Density Liquids

The pipette is able to compensate for solutions of density much different than water by setting the volume slightly higher or lower than required.

For liquids less dense than water, compensate by setting the volume meter lower than the required value.
For liquids denser than water, set the volume meter higher that the required value.

Pipetting 80µl of serum using 100µl pipette.

If the second measurement is not accurate enough, make another slight volume adjustment until the measurements are as desired. (See steps 1- 6 above.)


Pipettes: Clean periodically with a soap solution or 60% isopropanol. Rinse in distilled water and let dry.

Seals: These are maintenance-free.

Pistons: Should be lubricated lightly with silicone grease when cleaned or replaced.

Sample contamination caused by the liquid entering the pipetter can be removed after the pipette has been disassembled.


The entire pipette can be sterilized by steam-autoclaving (121°C, 15 psi, 15 minutes) The pipette will need to be recalibrated after autoclaving. Let the pipette dry at room temperature before use. Any discoloration which may occur has no effect on the function of the pipette.


Problem Cause Solution
Droplets on inner wall
of pipette tip
Contaminated/dirty pipette tip Use a new pipette
Pipette is dripping and/or volume pipetted is incorrect Tip is loose Press tip onto end of the shaft firmly
An unsuitable tip has been used Use correct tip
Tip is removed too quickly Pull tip slowly out of the liquid
Piston is contaminated Clean and lightly lubricate the piston
Piston/seal is damaged Replace piston/seal
Assemply is loose Tighten the pipette assembly nut
Control button jams and does not move smoothly Piston is contaminated Remove piston/seal from the pipette. Clean piston/seal with DI water. Replace piston/seal if necessary.
Liquid has entered the nose cone and dried Remove piston from the pipette lower part, rinse out lower part with warm water, then rinse with DI water and dry.

NOTE: When removing the shaft from the pipette body, be sure the spring, seal and o-ring do not fall off the piston.

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UV Stable Adjustable Pipetters