EMS Technical Data Sheets

SepCon™ Spin Vial for Microparticle Separation & Concentration

EMS Catalog #76042-22 through 76042-25

SepCon Spin Vial for Nanoparticle Separation & Concentration

Cut-Off Sizes 0.5, 1.0 µm
Capacity 20-500 µL

SepCon Spin Vials are for research use only and not for use in diagnostic of clinical applications.


Required Equipment

SepCon Spin Vial for Nanoparticle Separation & ConcentrationParticle Separation

  1. Remove SepCon vial from supplied microcentrifuge tube and pipette sample solution (20-500 µL) into upper portion of vial.
  2. Place SepCon vial in microcentrifuge tube and align the vial's ridge with cap (A).
  3. Place microcentrifuge tube in rotor with the vial's ridge pointed to the center of the rotor.
  4. Operate centrifuge at up to 600 x g for 20 minutes or until the sample has cleared the upper portion of the vial.

Particle Recovery

  1. Pipette a sufficient volume of the desired elution buffer into the upper portion of the vial; for example, 100-200 µL.
    Note: Optionally wash the membrane bound material with a suitable buffer by repeating the separation protocol above.
  2. To recover material from the membrane surface, pipette the elution buffer carefully and successively across the membrane.
  3. Aspirate all the elution buffer via pipette and transfer to a new microcentrifuge tube

Filter Recovery (Optional)

    1. Holding the SepCon vial and using tweezers or similar implement, apply pressure on the lower cap part to force it to separate from the upper vial part.
    2. Once the cap and vial are separated, carefully recover the filter from the inside of the cap with flat-sided tweezers.
    3. Transfer the filter to a microscope slide or other substrate for further analysis.
      Note: If imaging with short working distance lenses, then remove the square silicone gasket from the filter.
Operating Parameters
Initial Sample Volume 20-500 µL
Maximum Centrifugal Force 600 x g
Recommended Starting Concentration <0.5% solids



SepCon Spin Vials are fabricated and packaged under clean conditions, but are not guaranteed sterile. SepCon spin vials can be sterilized by steam autoclaving, ethylene oxide, hydrogen peroxide, isopropanol or ethanol solutions. Do not sterilize with sodium hydroxide (NaOH).

Chemical Stability

The membranes are made of silicon nitride (SiN), which is significantly more stable than conventional polymeric membranes in most acids, alcohols and solvents. Avoid prolonged exposure to significantly basic solutions (>10.0 pH) as membrane damage may occur. While SiN is stable against most solvents, the polyethylene vial and polypropylene microcentrifuge tube may not be suitable for prolonged exposure. Reference a compatibility database prior to use.

Device Storage

SepCon spin vials should be stored at room temperature in a clean and dry location and used within 1 year from ship date.

Physical Properties
Membrane Composition Silicon Nitride (SiN)
Thickness 400 nanometers
Pore Size Cut-Off 500, 1000 nanometers
Porosity 10% (500 nm), 16% (1000 nm)
Surface Charge Neutral to slightly negative
Tube & Vial Material High-density Polypropylene
Gasket Material Platinum-cured Silicone, USP VI

Online Ordering

SepCon™ Spin Vials