The combined TEM test specimen is made of a combination of small gold islands on a perforated, carbon film and a light deposition of graphitized carbon. The perforations on the film serve to indicate any defects by observing through the over focused Fresnel fringe.
The graphitized carbon particles, as explained above, provide a lattice spacing of 0.34nm.
Note: This should be observed where the particles overlap the holes on the film.
The gold islands provide rough measurements of particle to particle resolution. Some of them will be oriented in such a way that produces gold lattice images.