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EMS Technical Data Sheets
SmartGlow™ Safe Green Pre-Stain
EMS Catalog #87058-05
SmartGlow™ Safe Green Pre-Stain is a non-carcinogenic alternative to Ethidium Bromide. It is excellent for nucleic acid electrophoresis and purification applications. You can use SmartGlow™ Safe Green Pre-Stain for pre-staining or post-staining agarose gels. The stain emits green fluorescence when bound to dsDNA or ssDNA and emits red fluorescence when bound to RNA. SmartGlow™ Safe Green Pre-Stain has two excitation peaks at approximately 290 nm (UV light) and 490 nm (blue light).
Instructions
- Prepare 100 ml of agarose or polyacrylamide solution.
- Add 5µl of SmartGlow™ Safe Green Pre-Stain to the gel solution.
- Mix gently and make sure there are no air bubbles in the mixture.
- Allow the solution to cool to about 60-70°C, then pour the gel(s).
- For enhanced results, you can add (optional) 5µl of SmartGlow™ Safe Green Pre-Stain per 100 ml of the running buffer – adding the stain to the running buffer can help in increasing sensitivity and detecting small quantities of nucleic acid.
- After the separation is complete, view the gel using a UV or blue light illuminator.
FAQs
Q: What is the shipment/storage temperature?
A: For the maximum performance and stability, SmartGlow should be stored at 4°C and in the dark.
Q: What is the shelf life of the SmartGlow DNA stain?
A: The shelf life is a minimum of 2 years when stored properly at 4°C.
Q: Can SmartGlow stain be diluted?
A: SmartGlow solution can be diluted in the ultrapure water.
Q: Can SmartGlow be used with TAE and TBE buffers?
A: Yes. When running agarose gel, the electrophoresis buffer may be either TAE or TBE.
Q: Which filters (wavelength) can be used for SmartGlow imaging?
A: The filters designed for green dyes (like GF/SYBR green filter) are the most sensitive. However, EtBr filters and even UV-light without any filter can be used.
Q: Does SmartGlow work with low concentrations of DNA?
A: Yes, it works well with low concentrations of DNA, however, smaller fragments (less than 300 bp) may not be as bright as the larger ones (post-staining).
Q:Does SmartGlow interfere with downstream applications?
A: Avoid leaving SmartGlow in downstream solution. You may purify the DNA solution with a purification kit.
Q:Can SmartGlow be used for pulse field gel electrophoresis?
A: It is not recommended to use SmartGlow for pulse field gel electrophoresis.
Q: Can SmartGlow be used in Southern blots?
A: Yes, SmartGlow is compatible with Southern blotting experiments.
Q: Does the stain interfere with recovery of DNA from the stained gel once it is run?
A: No, the stain should not cause any problems for further experiments with that DNA.
Q: Does SmartGlow stain penetrate latex gloves?
A: SmartGlow does not penetrate latex gloves.
Q: How can SmartGlow be disposed after use?
A: SmartGlow does not create any toxic waste. Therefore, it can be disposed according to laboratory regulations.
Product Information
SmartGlow™ Safe Green Pre-Stain