EMS Technical Tips

Adherence of LR White Sections to Glass Slides for Silver Enhancement Immugold Labeling

A continuing problem in immunogold labeling of 1 micron thick sections from blocks embedded in the hydrophilic acrylic resin LR White for Light Microscopy is the lack of adherence of the sections to the glass slide during the silver enhancement. This problem can be solved by using a 2% formvar solution to coat the glass slide.


Slater, M. (1989) Adherence of LR White Sections to GlassSlides for Silver Enhancement. Immunogold Labeling. Vol. 65, No. 6 Stain Technology. pp. 297-299