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EMS Technical Tips
A Methylene Blue-Azure II Stain
Superior Methylene Blue-Azure II Stain for Semithin Sections
The most common stain used in the electron microscopy lab for thick sections is toluidine blue. Unfortunately its general lack of polycromasia makes it unsuitable for photomicrography. Much better results are obtained for general work as well as for photomicrography (especially with the use of filters for black and white film) with the methylene blue, azure II combinations.
Reference:
Hildegard H. Crowley, Dept. of Biological Sciences, University of Denver, Denver, CO. 80208