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- Prepmaster™ Specimen Preparation Robot
- TEM Grids
- TEM Window Grids
- Omniprobe Nanomanipulation Systems
- K-kit Wet "Liquid" TEM Kit
- Specimen Mounts
- SEM Specimen Holders
- Index and Finder SEM Grids
- SEM for Forensics
- SEM Sample Preparation Station Materials
- Cryogenic Personal Protection Equipment
- Cryo Dewars & Flasks
- Cryogenic Grids & Accessories
- Cryogenic Vials & Racks
- Cooling Chambers & Ice Baths
- Prepmaster™ Specimen Preparation Robot
- Laboratory Microwave Ovens
- LYNX II Automated Tissue Processor
- EMS Poly III
- Microtomes
- Tissue Slicers
- Rapid Immersion Freezer
- Heaters & Chillers
- SEM Cooling Stage
- Glow Discharge Systems
- Sputter Coaters & Carbon Coaters
- Stages
- Freeze Dryers
- Critical Point Dryers
- Cryo-SEM Preparation System
- Specimen Transfer Systems
- Decontaminators
- Desiccators
- Centrifuges
- Dry Baths
- Stirrers, Hot Plates
- Vortexers & Magnetic Mixers
- Rotators & Rockers
- Ovens & Incubators
- Vibration Isolation
- Air Sampling
- Vacuum Pumps
EMS Technical Tips
Cryo-Protectants
Studies have shown that Glycerol and Dimethyl Sulfoxide give good structural preservation for cryo-protectants, but they also cause gross physical damage to cells, and they should not be used in microanalytical studies. For better results, high-molecular-weight nonpenetrating cryoprotectants such as polyvinylpyrolidone (PVP) and hydroxyethyl starch (HES) can give good morphological preservation, and only cause minimal perturbation to the functional physiology of the tissue.
References:
Sauberman, A.J., Riley, W., and Echlin, p. 1977a SEM/1977/I,II T Research Institute, Chicago, Illinois, p.347
Technical Tips 