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EMS Technical Tips
Ultra Small ImmunoGold Reagents Application Instructions
Detailed information is provided on the package inserts.
Electron Microscopy Labeling
For post-embedding labeling, incubate thin sections by placing grids on drops of reagent arrayed on a sheet of Parafilm™. The use of nickel grids is recommended, especially if silver enhancement procedures are intended.
For pre-embedding labeling, float specimens in dilute reagent on a rocking table.
For more information on post and pre-embedding labeling;
- Post-embedding Immuno Incubation Protocol
- Pre-embedding Immuno Incubation Protocol
- Ultra Small Gold Reagents and R-Gent SE-EM in Pre-embedding single and double labeling
Light Microscopy Slide or Coverslip Labeling
Apply a few drops of dilute reagent to cover the specimen.
Recommended Incubation Solution:
PBS,
(10 mM Phosphate buffer, 150 mM NaCl)
0.1-0.2 % AURION BSA-c™
15 mM NaN3
pH 7.4
Additional Information
"Ultra Small Gold Reagents and R-Gent SE-EM in Pre-embedding Single and Double Labeling" Hong Yi and Jan L.M. Leunissen
"Optimized Immuno Labeling using AURION BSA-c™ and AURION Blocking Solutions" by J.L.M. Leunissen and P.F.E.M. van de Plas
For additional information please see the Aurion Immunogold Newsletter and Protocol sections.