EMS Technical Tip

Conventional ImmunoGold Reagents Application Instructions

Detailed information is provided on the package inserts..

On-Grid Labeling

For most applications grids are floated on top of drops of dilute reagent displayed on a sheet of Parafilm™. The use of gold or nickel grids is recommended.

Whole mount and intact cell labeling

Specimens are kept floating in dilute reagent on a rocking table.

Recommended Incubation Solution:

(10 mM Phosphate buffer, 150 mM NaCl),
0.1-0.2 % AURION BSA-C™
15 mM NaN3
pH 7.4

Additional Information

For additional information please see the Aurion Immunogold Newsletter and Protocol sections