Detailed information is provided on the package inserts..
For most applications grids are floated on top of drops of dilute reagent displayed on a sheet of Parafilm™. The use of gold or nickel grids is recommended.
Specimens are kept floating in dilute reagent on a rocking table.
(10 mM Phosphate buffer, 150 mM NaCl),
0.1-0.2 % AURION BSA-C™
15 mM NaN3