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Application Instructions for Solutions with BSA, CWFS, and Normal Sera

The following recipes have been tried succesfully by researchers in the field and by Aurion:

Blocking Solution for protein A or G reagents

Phosphate buffered saline
(10mM Phosphate buffer, 150 mM NaCl)
5 % BSA
0.1% CWFS Gelatin
15 mM NaN₃
pH 7.4

Blocking Solution for secondary antibody reagents

Phosphate buffered saline
(10mM Phosphate buffer, 150 mM NaCl)
5 % BSA
0.1% CWFS Gelatin
5-10 % normal serum
(same species as in the secondary antibody reagent)
15 mM NaN₃
pH 7.4

Incubation solution for Conventional Immunogold Reagents

Phosphate buffered saline
(10mM Phosphate buffer, 150 mM NaCl)
0.1-0.2 % BSA
0.1% CWFS Gelatin
1-5 % normal serum
(same species as in the secondary antibody reagent)
15 mM NaN₃
pH 7.4

Incubation solution for Ultra Small Immunogold Reagents

Phosphate buffered saline
(10mM Phosphate buffer, 150 mM NaCl)
0.8 % BSA
0.1% CWFS Gelatin
1-5 % normal serum
(same species as in the secondary antibody reagent)
15 mM NaN₃
pH 7.4

Additional Information

For additional information please see the Aurion Immunogold Newsletter and Protocol sections

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