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The following recipes have been tried succesfully by researchers in the field and by Aurion:
Phosphate buffered saline
(10mM Phosphate buffer, 150 mM NaCl)
5 % BSA
0.1% CWFS Gelatin
15 mM NaN3
pH 7.4
Phosphate buffered saline
(10mM Phosphate buffer, 150 mM NaCl)
5 % BSA
0.1% CWFS Gelatin
5-10 % normal serum
(same species as in the secondary antibody reagent)
15 mM NaN3
pH 7.4
Phosphate buffered saline
(10mM Phosphate buffer, 150 mM NaCl)
0.1-0.2 % BSA
0.1% CWFS Gelatin
1-5 % normal serum
(same species as in the secondary antibody reagent)
15 mM NaN3
pH 7.4
Phosphate buffered saline
(10mM Phosphate buffer, 150 mM NaCl)
0.8 % BSA
0.1% CWFS Gelatin
1-5 % normal serum
(same species as in the secondary antibody reagent)
15 mM NaN3
pH 7.4
For additional information please see the Aurion Immunogold Newsletter and Protocol sections