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- Prepmaster™ Specimen Preparation Robot
- TEM Grids
- TEM Window Grids
- Omniprobe Nanomanipulation Systems
- K-kit Wet "Liquid" TEM Kit
- Specimen Mounts
- SEM Specimen Holders
- Index and Finder SEM Grids
- SEM for Forensics
- SEM Sample Preparation Station Materials
- Cryogenic Personal Protection Equipment
- Cryo Dewars & Flasks
- Cryogenic Grids & Accessories
- Cryogenic Vials & Racks
- Cooling Chambers & Ice Baths
- Prepmaster™ Specimen Preparation Robot
- Laboratory Microwave Ovens
- LYNX II Automated Tissue Processor
- EMS Poly III
- Microtomes
- Tissue Slicers
- Rapid Immersion Freezer
- Heaters & Chillers
- SEM Cooling Stage
- Glow Discharge Systems
- Sputter Coaters & Carbon Coaters
- Stages
- Freeze Dryers
- Critical Point Dryers
- Cryo-SEM Preparation System
- Specimen Transfer Systems
- Decontaminators
- Desiccators
- Centrifuges
- Dry Baths
- Stirrers, Hot Plates
- Vortexers & Magnetic Mixers
- Rotators & Rockers
- Ovens & Incubators
- Vibration Isolation
- Air Sampling
- Vacuum Pumps
EMS Technical Tips
Poly-L-Lysine Coating of Slides
Poly-L-Lysine with a molecular weight greater than 150,000 (150,000-300,000) should be used. To make a stock solution: Take 0.1% Poly-L-Lysine in distilled water and store it in a dispensing vial and freeze it at -20°C. A drop (10-15ml) is dispersed at the end of a clean glass slide and smeared along its length with the end of another clean glass slide in the same way as blood film. Make sure that the film of Poly-L-Lysine is thin enough for interference colors to be seen as it is being smeared. The slide is ready to use within minutes of drying.
If the Poly-L-Lysine is properly applied , it will not affect immunostaining, but it may cause precipitation in some silver impregnation methods. Therefore, It should be avoided if immunogold-silver methods are to be used.