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Electron Microscopy Sciences

Illuminators, Magnifiers, Microscopes, & Graticles

arrow13NIGHTSEA® Fluorescence Viewing Systems

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Image GalleryEclipse MicroTentarrow12Eclipse MicroTent

  • Tent
  • Tent
  • Tent

The patented (US Pat. No. 10,175,467) Eclipse MicroTent™ is a unique product for fluorescence microscopy that provides local darkness around conventional laboratory stereo microscopes. Fluorescence microscopes are kept in dark rooms for good reason - fluorescence can be weak and in many cases it can be difficult to see well if there is any ambient light. Microscopes may be on lab benches in shared spaces, near windows, or in other difficult-to-darken locations such as in the field. Turning off overhead lights can help but inconveniences others.

The Eclipse Microtent™ creates local darkness around a microscope while still providing easy access to the sample stage and the focus and zoom controls. It is designed for stereo microscopes but could potentially be used with many varieties of compound microscopes.

Features

  • Opening for the microscope oculars with elastic sleeve to minimize light entry
  • Large front flap provides easy access to the sample stage and can fasten open
  • Arm slots on sides to provide access to focus and zoom controls
  • User-customizable feedthrough patches to provide additional penetrations for camera port, power cords, CO2 lines, or other features as you need
  • Tru-Block™ Eye Shields included with every Eclipse MicroTent
  • Folds flat for storage
  • Dimensions: 46 x 30 x 50 cm (18 x 12 x 20 in.)

Ordering Information

SFA-TENT Eclipse MicroTent each 325.00 Add to Cart

Technical Data SheetEye Shieldsarrow12Eye Shields

Light entering your eyes from the side can interfere with what you want to see in microscopy in general, and fluorescence microscopy in particular. Eye cups are available, but the standard ones don't extend far from the microscope and don't do a good job as ambient light increases. Our soft, molded rubber high-sided microscope eye shields are the answer. The tall wings extend up far enough to truly shield your eyes from any level of ambient light and eliminate distractions so that you can see your subject better. Two pairs (one Standard, one Compact) are included with every Eclipse MicroTent™, and you can also purchase them separately.

The Tru-Block Eye Shields are available in two sizes

  • Standard - fits 36 - 45mm (1.45 - 1.75")
  • Compact - fits 28 - 37mm (1.10 - 1.46")

Ordering Information

SFA-EYE-S Tru-Block Eye Shields - Standard set 18.00 Add to Cart
SFA-EYE-C Tru-Block Eye Shields - Compact set 18.00 Add to Cart

Modular White Light Headarrow12Modular White Light Head

This is an extremely convenient option for general illumination and as a focusing aid for fluorescence imaging. Now if you are using the NIGHTSEA system for fluorescence you do not need a separate white light source. Just exchange the fluorescence excitation light head module for the white-light module in a matter of seconds.

Ordering Information

SFA-LH-WH Modular White Light Head each 230.00 Add to Cart
Modular Infrared Light Head
850nm IR Light Head
Modular Infrared Light Head
850nm IR Light Head on SFA lamp base

arrow12Modular Infrared Light Head

Researchers sometimes need an infrared light source for non-intrusive observation of behavior. And sometimes the subjects are small and you need to make the observations with a stereo microscope.

The SFA-LH-IR850 is an 850nm light source that is compatible with the NIGHTSEA Model SFA Stereo Microscope Fluorescence Adapter. The light head plugs directly into the lamp base supplied with the SFA system. You can acquire the light head as a supplement to the SFA system that you already own for fluorescence observation, or you can acquire it in combination with a lamp base as a stand-alone device.

The light head incorporates a high power 850nm LED and a medium beamwidth diffusing lens to create a smooth illumination area. The output is centered at 850nm with a FWHM (full width at half maximum) of approximately 50nm. There is virtually no emission at wavelengths shorter than 750nm.

  • Conventional camera under normal room light
  • Test target for IR Light Head, Infrared

  • Click to enlarge

The photo on the left above was taken with a conventional camera under normal room light, and with the IR light head illuminated at full power, directed at the target center from a distance of approximately 10cm (4 in.). The illumination spot is not visible either to this camera or to the naked eye. The photo on the right was made under the same lighting conditions, but with a camera that had been modified to image only infrared light.

Please note that infrared light is not visible to the human eye and to many cameras. You will need an appropriate camera to record the observations.

Ordering Information

SFA-LH-IR850 Modular Infrared Light Head each 250.00 Add to Cart
SYS-IR850 Modular Infrared Light Head w/DIM Base each 915.00 Add to Cart

Battery and Chargerarrow12Battery and Charger

Compact battery pack that can run the Stereo Microscope Fluorescence Adapter (SFA) all day long. The battery enables truly portable operation so that you can set up demos anywhere or explore fluorescence in the field, without having to worry about plugging into the power grid.

Just plug the battery into the SFA base instead of the usual power connector. The battery has been tested at over 8 hours of continuous operation, and it will last even longer if you turn the system off when you don't need it. Plug it into the included charger overnight to recharge.

Specifications

Battery Type Nickel Metal Hydride (NiMH)
Capacity 12V, 3.8 Ah
Dimensions 11.4 x 8.9 x 5.6 cm (4.5 x 3.5x 2.19 in)
Weight 0.7 kg (1 lb 9 oz)
Operation Duration 8 hours continuous
Charge time Overnight
Charger 50/60 Hz, 110/220V (US type plug)

SFA-BATT Battery and Charger each 280.00 Add to Cart


SFA + Eclipse MicroTent + Battery = Fluorescence Everywhere

Combine the battery with the Eclipse MicroTent and you not only don't need a place to plug in, you don't even need to be in the dark! We have used this combination to do fluorescence microscopy at a beach in the middle of the day, in the desert at high noon, and more. Fluorescence can be found everywhere, and now you have the tools to go there.

arrow12SFA A LA CARTE

Need an extra, not a set? Order from here

arrow12SFA Light Heads

SFA-LH-UV Light Head, Ultraviolet each 400.00 Add to Cart
SFA-LH-VI Light Head, Violet each 250.00 Add to Cart
SFA-LH-RB Light Head, Royal Blue each 250.00 Add to Cart
SFA-LH-CY Light Head, Cyan each 250.00 Add to Cart
SFA-LH-GR Light Head, Green each 250.00 Add to Cart

arrow12SFA Barrier Filters

SFA-BF-UV Barrier Filter, Ultraviolet each 208.00 Add to Cart
SFA-BF-VI Barrier Filter, Violet each 208.00 Add to Cart
SFA-BF-RB Barrier Filter, Royal Blue each 208.00 Add to Cart
SFA-BF-RB-GO Barrier Filter, Green Only each 208.00 Add to Cart
SFA-BF-CY Barrier Filter, Cyan each 208.00 Add to Cart
SFA-BF-GR Barrier Filter, Green each 208.00 Add to Cart

arrow12SFA Filter Shields

SFA-SH-UV Filter Shield, Ultraviolet each 105.00 Add to Cart
SFA-SH-VI Filter Shield, Violet each 105.00 Add to Cart
SFA-SH-RB Filter Shield, Royal Blue each 105.00 Add to Cart
SFA-SH-RB-GO Filter Shield, Green Only each 105.00 Add to Cart
SFA-SH-CY Filter Shield, Cyan each 105.00 Add to Cart
SFA-SH-GR Filter Shield, Green each 105.00 Add to Cart

arrow12SFA Adapters

SFA-AD Adapter each 168.00 Add to Cart
SFAZ-AD Leica EZ4 Adapter each 168.00 Add to Cart
SFA-XL-AD Oversize Adapter each 168.00 Add to Cart

arrow12SFA Bases

SFA-BASE Standard Base each 415.00 Add to Cart
SFA-BASE-DIM DIM Base each 665.00 Add to Cart
SFA-BASE-PULSE PULSE Base each 665.00 Add to Cart
Single SFA light head attached to the gooseneck by a Light Head Cable (LHC)
Single SFA light head attached to the gooseneck by a Light Head Cable

Two SFA light heads attached to the gooseneck by a Dual Light Head Cable (DLHC)
Two SFA light heads attached to the gooseneck by a Dual Light Head Cable

Two SFA Light Heads mounted to the NIGHTSEA adapter ring with Light Head Hangers
Two SFA Light Heads mounted to adapter ring with Light Head Hangers

Light head hanger configured for LWD (left) and SWD microscopes
Light head hanger configured for LWD (left) and SWD microscopes

Long working distance SFA Light Head Hanger at highest position
Single light head hanger mounted on microscope

arrow12Light Head Hanger System for the Stereo Microscope Fluorescence Adapter

The Light Head Hanger system is an accessory for the NIGHTSEA Model SFA Stereo Microscope Fluorescence Adapter. It is a combination of power extension cables and a custom made holder that enables you to take the fluorescence excitation light heads off the gooseneck and mount them directly on the SFA adapter ring. In addition, it provides the capability to drive two light heads from one lamp base.

The extension cables plug into the gooseneck lamp base and can bring power to either one or two light heads.

The Light Head Hanger mounts to the end of any of the thumbscrews on the SFA adapter ring. It can be configured for use with either long or short working distance microscopes.

  • Long working distance (LWD) – 6.5 cm (2.56") and greater
  • Short working distance (SWD) – 6.5-4.6 cm (2.5-1.8")

There are two main potential benefits with this accessory.

1) Increase excitation intensity without buying a second lamp base. Without this system, if you wanted to increase the fluorescence excitation intensity of the SFA, you would need to buy a second light head and lamp base. This would take up additional real estate on your laboratory bench. With the Light Head Hanger system you can power two light heads from one base and mount them both directly to the NIGHTSEA adapter ring.

"We had some issue detecting red fluorescent proteins with some of our weaker transgenic zebrafish lines, but by shining two LED lights onto the same embryo, most of our weakest GFP and mCherry transgenic lines can now be detected. One light head was fine for reasonably bright specimens, and with two lights, the fluorescence is nearly the same as traditional stereomicroscopes priced $15-20K."

2) Reduce the chance of accidentally bumping the light. Taking the light head off the gooseneck and mounting it directly to our microscope adapter ring reduces the chance of the light head being bumped so that it is not illuminating the subject on the microscope stage to best effect. This might be especially valuable if you are using the system for outreach demonstrations and multiple people will be working around the microscope.

Ordering Information

SFA-LHC Single Light Head Cable each 35.00 Add to Cart
SFA-DLHC Dual Light Head Cable each 65.00 Add to Cart
SFA-LHH Light Head Hanger each 95.00 Add to Cart
SFA-HK Hanger Kit for Single Light Head:
Includes Single Light Head Cable and one Light Head Hanger
each 125.00 Add to Cart
SFA-DHK Hanger Kit for Dual Light Heads:
Includes Dual Light Head Cable and two Light Head Hangers
each 245.00 Add to Cart
Note: If you are ordering a Dual kit to power two light heads at once for increased intensity, you will need to have two light heads of the same color. Above prices do not include the cost of an additional light head.
The default configuration is for systems with the BNC connector on the lamp base and the light head. Cables are available for the pre-2015 V1 connector on request.

arrow12Mobile Extension Kit

  • Hand grip with light head enclosure; extension cable; and barrier filter glasses
  • Hand grip with Royal Blue light head installed and connected to the power cable, and the Royal Blue filter glasses
  • Inspecting artwork with the SFA Mobile Extension Kit

The Mobile Extension Kit provides added versatility to your SFA microscope fluorescence adapter system by enabling you to use the light head as a handheld light source. Do you work with both macro and micro subjects? Do you want to screen larger specimens before dissection or other microscope work? Can't fit everything under the microscope? Then the SFA-MEK Mobile Extension Kit is a way to get more use out of your system.

The kit includes three components

  • Rubber-coated hand grip with wrist lanyard and custom made enclosure that holds an SFA Light Head securely. The slots on the side of the enclosure provide for cooling and also let you see the color code of an installed light head.
  • Lightweight extension cable.
  • Barrier filter glasses.

To use the kit just take the light head off the SFA gooseneck base, connect it to one end of the extension cable, and attach the other end of the cable to the gooseneck. Insert the light head in the holder and screw down the thumbscrew to secure it in place. Put on the filter glasses that correspond to the light head color and you're ready to go! If you want to use the Mobile Extender with more than one excitation wavelength you can add additional barrier filter glasses at any time.

If you want to use the Mobile Extension Kit with the SFA system you already own, then the SFA-MEK is all you need.

If you want to add additional excitation/emission combinations to use only for handheld operation and not for your microscope, you would need a Light Head plus a pair of Barrier Filter Glasses for each color.

Ordering Information

SFA-MEK-UV Mobile Extension Kit, UV Glasses each 225.00 Add to Cart
SFA-MEK-VI Mobile Extension Kit, VI Glasses each 225.00 Add to Cart
SFA-MEK-RB Mobile Extension Kit, RB Glasses each 225.00 Add to Cart
SFA-MEK-RB-GO Mobile Extension Kit, RB-GO Glasses each 225.00 Add to Cart
SFA-MEK-CY Mobile Extension Kit, CY Glasses each 225.00 Add to Cart
SFA-MEK-GR Mobile Extension Kit, GR Glasses each 225.00 Add to Cart

Technical Data SheetSwitch Box Kitarrow12Switch Box Kit

The Switch Box Kit enables you to mount two different wavelength excitation light heads on your microscope and switch between them easily. You will still need to manually change the barrier filter, but it eliminates the need to swap out light heads on the gooseneck.

The Switch Box Kit is available only for SFA systems that use the BNC light head connector, and not the older V1 connector.

Ordering Information

SFA-SWK Switch Box Kit, includes BNC switch box, 3 BNC cables, 2 LH Hangers kit 350.00 Add to Cart

arrow11Lamp Base Light Control Options

The SFA lamp base is available in three versions: Standard, DIM, and PULSE. Standard lamp bases have a simple OFF/ON operation. DIM lamp bases feature an OFF/ON/DIM switch to change the intensity of the light. PULSE lamp bases incorporate a BNC connector that accepts a voltage signal to control the excitation source ON/OFF.

Only one control option is available per base. This DIM and PULSE options can be purchased as part of a new system, as a retrofit to an existing base, or in a stand-alone lamp base purchased a la carte.

Dimmer switch option on SFA

Target at max and min excitation

arrow12NIGHTSEA DIM Option

The DIM option adds a combined switch and dimmer control.

When you first turn the unit on it is at full power.

As you rotate the switch the intensity decreases, reaching about 30% power at the minimum setting.

You have finer control in the brighter portion of the adjustment range.

An intensity indicator (1-10) makes it easy to record and repeat preferred settings.

Ordering Information

SFA-BASE-DIM-R Dim Option - Retrofit each 275.00 Add to Cart
BNC input connector for external controlBNC input connector for external control

arrow12NIGHTSEA PULSE Option

The PULSE option adds a BNC connector on the rear of the base that accepts a user-supplied voltage input to turn the light on and off. Some possible applications

  • External control (computer, function generator, etc.) for precise illumination timing for photoactivation, behavioral experiments
  • PWM input for light dimming
  • Footswitch for hands-free operation - contact EMS for details

Specifications

  • Voltage input - 2.8 - 6.0 VDC for ON, <0.6VDC OFF
  • Maximum operation frequency - 10kHz
  • BNC cable not included

Ordering Information

SFA-BASE-PULSE-R PULSE Option - Retrofit each 275.00 Add to Cart
NIGHTSEA Xite flashlight
Xite flashlights - 5 excitation wavelengthsGoBe NIGHTSEA light with remote phosphor cap in place
Xite-RB and glasses with a fluorescent mineral

arrow11Xite Fluorescence Flashlight System

View fluorescence in the lab or the field! The Xite™ Fluorescence Flashlight System includes single-wavelength fluorescence excitation flashlights available with any of the five wavelengths in the NIGHTSEA line, paired with matching barrier filter glasses for maximum viewing contrast. Genotyping your transgenic organisms, screening cell cultures, inspecting parts with fluorescent penetrants, finding fluorescing critters in the field, ... the Xite line provides what you need.

Features

  • Tightly focused for a high intensity illumination spot
  • Two power levels, plus unique flashing mode and battery status indication
  • Add-on diffuser for a broader, smoother beam
  • Each light paired with matching barrier filter glasses
  • Compact and lightweight
  • Rechargeable high capacity lithium ion battery

Included with each system

  • Fluorescence excitation flashlight (your choice of one wavelength) with lanyard
  • Matching barrier filter glasses with microfiber case for storage and cleaning
  • Diffuser cap
  • High capacity lithium ion battery
  • Battery charger and USB charging cord
  • Instruction sheet
  • Padded foam shipping/carry/storage case

Diffuser

Add-on 15° diffuser cap provides a wider, softer beam.

Click an image to enlarge
  • NIGHTSEA Xite light with push-on diffuser
  • Xite illumination spot, no diffuser
  • Xite illumination spot, with diffuser

Technical Data Sheet Flashing mode for enhanced detection in ambient light

As bright as fluorescence may appear when you use an intense light source to view it in conditions of darkness, in most cases it actually tends to be a relatively weak effect that is easily masked by other light. If you try to find fluorescence when there is moderate ambient light (room light, sunlight, etc.), any fluorescing subject will respond to the excitation, but the response may be too weak to notice easily.

But there's a trick - by making the excitation light source blink repetitively (a strobe effect), any fluorescence will blink at the same rate, while the illuminated background will not. This flickering increases the apparent contrast and thus increases detectability.

Available wavelengths

Wavelength sets are named and color coded for the color of the excitation light, not the color of the emitted fluorescence.

Designation Excitation Barrier Filter Glasses
UV - Ultraviolet 360 - 380nm 415nm longpass
VI - Violet 400 - 415nm 450nm longpass
RB - Royal Blue 440 - 460nm 500nm longpass
RB-GO - Royal Blue, Green Only 440 - 460nm 500 - 560nm bandpass
CY - Cyan 490 - 515nm 550nm longpass
GR - Green 510 - 540nm 600nm longpass

With the Royal Blue (RB) excitation we offer two options for filter glasses - longpass and bandpass.

Flashlight specifications

  • Dimensions: 13.5 cm long x 3.2 cm diameter (5.3 in x 1.26 in)
  • Weight: 255g (9.0 oz) with battery
  • Note - the Xite flashlights are not suitable for underwater use

Ordering Information

Xite-UV Ultraviolet (360 - 380nm) excitation each 475.00 Add to Cart
Xite-VI Violet (400 - 415nm) excitation each 325.00 Add to Cart
Xite-RB Royal Blue (440 - 460nm) excitation with longpass filter glasses each 325.00 Add to Cart
Xite-RB-GO Royal Blue with green-only bandpass filter glasses each 325.00 Add to Cart
Xite-CY Cyan (490 - 515nm) excitation each 325.00 Add to Cart
Xite-GR Green (510 - 540nm) excitation each 325.00 Add to Cart
FG-UV Extra Filter glasses, Ultraviolet each 55.00 Add to Cart
FG-VI Extra Filter glasses, Violet each 55.00 Add to Cart
FG-RB-1 Extra Filter glasses, Royal Blue, style 1 each 55.00 Add to Cart
FG-RB-2 Extra Filter glasses, Royal Blue, style 2 each 55.00 Add to Cart
FG-RB-3 Extra Filter glasses, Royal Blue, style 3 each 55.00 Add to Cart
FG-RB-GO Extra Filter glasses, Green Only each 55.00 Add to Cart
FG-CY Extra Filter glasses, Cyan each 55.00 Add to Cart
FG-GR Extra Filter glasses, Green each 55.00 Add to Cart

NIGHTSEA Barrier Filter Glassesarrow11NIGHTSEA Barrier Filter Glasses

The Royal Blue filter glasses are available in a choice of three styles. Styles 1 and 2 fit over eyeglasses, Style 3 does not. All other glasses colors are
available in Style 2 only. Glasses meet ANSI Z87.1 impact standards for safety glasses. NOTE: Glasses Description refers to the excitation light source with
which they are to be used.

Ordering Information

FG-UV Filter glasses, Ultraviolet each 55.00 Add to Cart
FG-VI Filter glasses, Violet each 55.00 Add to Cart
FG-RB-1 Filter glasses, Royal Blue, style 1 each 55.00 Add to Cart
FG-RB-2 Filter glasses, Royal Blue, style 2 each 55.00 Add to Cart
FG-RB-3 Filter glasses, Royal Blue, style 3 each 55.00 Add to Cart
FG-RB-GO Filter glasses, Green Only each 55.00 Add to Cart
FG-CY Filter glasses, Cyan each 55.00 Add to Cart
FG-GR Filter glasses, Green each 55.00 Add to Cart

NIGHTSEA® FAQs arrow13arrow13