Prepared Staining Kits for Histology and Pathology

G. CYTOPLASMIC GRANULE STAINS

G.1 Pancreatic Islet Cell Stains

Mallory-Heidenhain Azan-Gomori's Modification, Islet Cells

For the staining of alpha, beta and D-cells of the islets of langerhans.

Stain results:

(Bouin's Fixation)

	Human Tissue	Guinea Pig
Alpha granules:	Red, bright	Orange - Tan
Beta granules:	Orange-Brown, dull	Red, fiery
D-cell granules:	Dark Blue	Deep Blue

References:

• Clark, G, (ed): Staining Procedures 3rd Ed.; Baltimore: Williams & Wilkins Co., c. 1973, p. 171
• Gomori, G.: "Studies on the cells of the Pancreatic islets." Anat. Rec. 74:439 (1939)

RT	26450-01	Azocarmine Staining Solution, 0.1%		250 ml	36.00	Add to Cart
RT	26450-02	Aniline-Alcohol, 1% Poison Pack		250 ml	17.00	Add to Cart
RT	26450-03	Ferric Ammonium Sulfate, 5%, Aqueous		500 ml	17.00	Add to Cart
RT	26450-04	Aniline Blue – Orange G Solution		500 ml	30.00	Add to Cart
RT	26450-05	Orange G, 0.2% in Alcohol		500 ml	32.00	Add to Cart

Maldonado's Method For Pancreatic Islet Cells

For the staining of alpha, beta, D- and Exocrine cells of the islets of langerhans.

Stain results:

(Bouin's Fixation without Acetic Acid)

	Human Tissue
Alpha granules:	Purple
Beta granules:	Violet Blue
D-cell granules:	Light blue with evident granules
Exocrine cells:	Grayish blue with red granules

References:

• Maldonado, R. and San Jose, H. Stain Techn., 42:11-13, 1967
• Luna, L.G., (ed.), Manual of Histologic Staining Methods of the AFIP, 3rd edition, McGraw, N.Y. p. 105, c. 1968

RT	26458-01	Phloxine 1% Aqueous Solution		500 ml	28.00	Add to Cart
RT	26458-02	Phosphotungstic Acid, 3% Aqueous Solution		500 ml	50.00	Add to Cart
RT	26458-03	Azure II Solution, 0.05%		500 ml	16.00	Add to Cart
RT	26458-04	Weigert's Iron Hematoxylin A		500 ml	27.00	Add to Cart
RT	26458-05	Weigert's Iron Hematoxylin B		500 ml	21.00	Add to Cart

G.2 Mast Cell Stains

Toren's Method for Mast Cells

Cytoplasmic granule stains: Mast cell stains.

Stain results:

Mast Cell Granules:		Deep Blue
Collagen & Cartilage:		Blue-Green
Muscle &Elastic Fibers:		Red
Fibrin:		Purple
Bone:		Green
Colloid, Keratin & Erythrocytes:		Orange Red to Orange

References:

• Toren, D.A.: Stain Techn., 38:249-250, 1963
• Luna, L., (ed.), Manual of Histologic Staining Methods of the AFIP, 3rd. ed., McGraw-Hill, New York, c. 1968, pp. 116-117

RT	26472-01	Giemsa Stain Solution		500 ml	70.00	Add to Cart
RT	26472-02	Acetic Acid, 0.5%, Aqueous		500 ml	16.00	Add to Cart
RT	26472-03	Pollack's Trichrome Solution in 50% Alcohol, 3A		100 ml	30.00	Add to Cart

G.3 Other Granule Stains

Methenamine Silver Method for Argentaffin Cells

For the staining of argentaffin granules of enterochromaffin cells.

Stain results:

Argentaffin Cells:		Black
Coarse connective tissue of the submucosa:		Variable amount of blackening by 3–31 ⁄2 hr. of impregnation
Granules of eosinophil leukocytes, nuclei, smooth muscle & surface epithelium:		show additional blackening after incubation beyond 3–31 ⁄2 hr.
Granules of mast cells:		Remain red after nuclei & reticulum are blackened

References:

• Clark, G.: Staining Procedures, Williams and Wilkins Co., Baltimore, 3rd Ed., c. 1973, p. 161.
• Gomori, G., Arch. Path., 45:48, 1948.
• Burtner, H.J. and Lillie, R.D., Stain Techn., 24:225-7, 1949.

RT	26481-01	Weigert's Iodine Solution		500 ml	32.00	Add to Cart
RT	26481-02	Sodium Thiosulfate, 5%, Aqueous		500 ml	22.00	Add to Cart
RT	26481-03	Silver Nitrate, 5%, Aqueous		250 ml	88.00	Add to Cart
RT	26481-04	Methenamine, 3%, Aqueous		500 ml	28.00	Add to Cart
RT	26481-05	Borate Buffer, pH 7.8		500 ml	22.00	Add to Cart
RT	26481-06	Gold Chloride, 0.1%, Aqueous		250 ml	32.00	Add to Cart
RT	26481-07	Saffranin O Solution, 0.1%		500 ml	32.00	Add to Cart

Fat and Lipid Stains